Expression Profiling of Cell Cycle Regulatory Proteins in Oropharyngeal Carcinomas Using Tissue Microarrays

Expression Profiling of Cell Cycle Regulatory Proteins in Oropharyngeal Carcinomas Using Tissue Microarrays

Author Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Nascimento, Fábio Dupart Autor UNIFESP Google Scholar
Fracalossi, Ana Carolina Cuzzuol Autor UNIFESP Google Scholar
Gomes, Thiago Simao Autor UNIFESP Google Scholar
Oshima, Celina Tizuko Fujiyama Autor UNIFESP Google Scholar
Franco, Marcello Fabiano de Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Aim: The aim of this study was to investigate the expressions of cell cycle regulatory proteins such as p53, p16, p21, and Rb in squamous cell carcinoma of the oropharynx and their relation to histological differentiation, staging of disease, and prognosis. Patients and Methods: Paraffin blocks from 21 primary tumors were obtained from archives of the Department of Pathology, Paulista Medical School, Federal University of Sao Paulo, UNIFESP/EPM. Immunohistochemistry was used to detect the expression of p53, p16, p21, and Rb by means of tissue microarrays. Results: Expression of p53, p21, p16 and Rb was not correlated with the stage of disease, histopathological grading or recurrence in squamous cell carcinoma of the oropharynx. Conclusion: Taken together, our results suggest that p53, p16, p21 and Rb are not reliable biomarkers for prognosis of the tumor severity or recurrence in squamous cell carcinoma of the oropharynx as depicted by tissue microarrays and immunohistochemistry.
Keywords Squamous cell carcinomas
tissue microarray
immunohistochemistry
p53
p16
p21
Rb
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho de Desenvolvimento Cientifico e Tecnologico
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 07/01228-4
Date 2010-05-01
Published in In Vivo. Athens: Int Inst Anticancer Research, v. 24, n. 3, p. 315-320, 2010.
ISSN 0258-851X (Sherpa/Romeo, impact factor)
Publisher Int Inst Anticancer Research
Extent 315-320
Origin http://iv.iiarjournals.org/content/24/3/315.full
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000279035400011
URI http://repositorio.unifesp.br/11600/43915

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