Imbalance of Tumor Suppression Genes Expression Following Rat Tongue Carcinogenesis Induced by 4-Nitroquinoline 1-Oxide

Imbalance of Tumor Suppression Genes Expression Following Rat Tongue Carcinogenesis Induced by 4-Nitroquinoline 1-Oxide

Author Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Fracalossi, Ana Carolina Cuzzuol Autor UNIFESP Google Scholar
Uatari, Silvia Akemi Autor UNIFESP Google Scholar
Oshima, Celina Tizuko Fujiyama Autor UNIFESP Google Scholar
Salvadori, Daisy Maria Favero Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Sao Paulo State Univ
Abstract This study was undertaken to investigate, by immunohistochemistry, the expression of some tumor suppressor genes such asp 16, p21 and Retinoblastoma (Rb) during 4-Nitroquinoline I-oxide induced rat tongue carcinogenesis. Male Wistar rats were distributed into three groups of 10 animals each and treated with 50 ppm 4NQO solution through their drinking water for 4, 12 or 20 weeks. Ten animals were used as negative control. Neither histopathological abnormalities were induced in the epithelium after 4 weeks of carcinogen exposure, not-statistically significant differences (p>0.05) in expression of all the tumor suppressor genes were found when compared to the negative control. However, the levels of Rb were increased (p<0.05) in pre-neoplastic lesions at 12 weeks following carcinogen exposure. In well-differentiated squamous cell carcinoma induced after 20 weeks of treatment with 4NQO, p16 and Rb were expressed in some tumor cells. Taken together, the results support the belief that the expression of Rb is closely event-related to malignant transformation and conversion of the oral mucosa, being a reliable biomarker linked to oral cancer pathogenesis.
Keywords P16
rat tongue mucosa
oral squamous cell carcinoma
4-nitroquinoline 1-oxide
malignant transformation
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 07/08210-3
FAPESP: 07/01228-4
Date 2009-11-01
Published in In Vivo. Athens: Int Inst Anticancer Research, v. 23, n. 6, p. 937-942, 2009.
ISSN 0258-851X (Sherpa/Romeo, impact factor)
Publisher Int Inst Anticancer Research
Extent 937-942
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000273199000009

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