The frequency of 844ins68 mutation in the cystathionine beta-synthase gene is not increased in patients with venous thrombosis

The frequency of 844ins68 mutation in the cystathionine beta-synthase gene is not increased in patients with venous thrombosis

Author Franco, Rendrik Google Scholar
Maffei, Francisco Humberto de Abreu Autor UNIFESP Google Scholar
Lourenco, Dayse Maria Autor UNIFESP Google Scholar
Piccinato, Carlos Google Scholar
Morelli, Vania Maris Autor UNIFESP Google Scholar
Thomazini, Isolete Google Scholar
Zago, Marco Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Background and Objectives. A frequent mutation in the cystathionine beta-synthase (CBS) gene (844ins68, a 68-bp insertion in the coding region of exon 8) was recently discovered. In the present study we Investigated this mutation as a candidate risk factor for venous thrombosis.Design and Methods. The prevalence of the 844ins68 CBS mutation was determined in 101 patients with objectively diagnosed deep venous thrombosis and in 101 healthy controls matched for age, sex and race. PCR amplification of a DNA fragment containing exon 8 of the CBS gene was employed to determine the genotypes, Additionally, Bsrl restriction enzyme digestion of the PCR products was performed in all samples from carriers of the insertion, to test for concurrent presence of a second mutation (T833C) in the CBS gene.Results. The insertion was found in 21 out of 101 patients (20.8%; allele frequency 0.109) and in 20 out of 101 controls (19.8%; allele frequency 0.114), yielding a relative risk for venous thrombosis related to the 844ins68 CBS mutation close to 1.0. In addition, the T833C GBS mutation was detected in all alleles carrying the 844ins68 CBS insertion, confirming the co-inheritance of the two mutations.Interpretation and Conclusions. Our findings do not support the hypothesis that the 844ins68 mutation in the CBS gene is a genetic risk factor for venous thrombosis. (C)1998, Ferrata Storti Foundation.
Keywords cystathionine beta-synthase
insertion variant
venous thrombosis
Language English
Date 1998-11-01
Published in Haematologica. Pavia: Ferrata Storti Foundation, v. 83, n. 11, p. 1006-1008, 1998.
ISSN 0390-6078 (Sherpa/Romeo, impact factor)
Publisher Ferrata Storti Foundation
Extent 1006-1008
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000077257000011

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