IDENTIFICATION OF SIALIC ACIDS ON THE CELL-SURFACE OF HYPHAE AND YEAST FORMS OF THE HUMAN PATHOGEN PARACOCCIDIOIDES-BRASILIENSIS

IDENTIFICATION OF SIALIC ACIDS ON THE CELL-SURFACE OF HYPHAE AND YEAST FORMS OF THE HUMAN PATHOGEN PARACOCCIDIOIDES-BRASILIENSIS

Author Soares, RMA Google Scholar
Alviano, C. S. Google Scholar
Angluster, J. Google Scholar
Travassos, Luiz Rodolpho Autor UNIFESP Google Scholar
Institution Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Abstract Paracoccidioides brasiliensis, the agent of paracoccidioidomycosis, when grown in a synthetic medium, expresses at the cell surface of both yeast and mycelial forms acidic glycoconjugates containing N-acetylneuraminic acid units. Sialic acids were extracted using mild hydrolytic conditions, and were identified by thin-layer and gas chromatography, standard colorimetry, reaction with periodate-resorcinol and mass spectrometry. Their surface location was inferred from fluorescent-lectin (Limulus polyphemus agglutinin) binding to whole cells abrogated by previous treatment with neuraminidase. Expression of sialic acids on virulent yeast forms of P. brasiliensis (3.7 X 10(6) residues per cell) may inhibit fungal phagocytosis during early infection, when the immunological response is still being built up.
Keywords SIALIC ACID
N-ACETYLNEURAMINIC ACID
PARACOCCIDIOIDES-BRASILIENSIS
YEAST FORMS
Language English
Date 1993-03-15
Published in Fems Microbiology Letters. Amsterdam: Elsevier Science Bv, v. 108, n. 1, p. 31-34, 1993.
ISSN 0378-1097 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 31-34
Origin http://www.sciencedirect.com/science/article/pii/037810979390483I
Access rights Closed access
Type Article
Web of Science ID WOS:A1993KU26800007
URI http://repositorio.unifesp.br/11600/43676

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