In Vitro Evaluation of Chloroaluminum Phthalocyanine Nanoemulsion and Low-Level Laser Therapy on Human Skin Dermal Equivalents and Bone Marrow Mesenchymal Stem Cells

In Vitro Evaluation of Chloroaluminum Phthalocyanine Nanoemulsion and Low-Level Laser Therapy on Human Skin Dermal Equivalents and Bone Marrow Mesenchymal Stem Cells

Author Primo, F. L. Google Scholar
Reis, M. B. da Costa Autor UNIFESP Google Scholar
Porcionatto, Marimélia Aparecida Autor UNIFESP Google Scholar
Tedesco, Antonio Claudio Autor UNIFESP Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Nanotechnology and tissue engineering are promising scientific fields in the development of advanced materials useful to human health. This article describes the preparation of a nanocarrier for the controlled release of a photosensitizer compound associated with low-level light therapy for skin wound healing treatment and applicable to other skin diseases. A biological model was used as an in vitro skin equivalent based on a three-dimensional culture of fibroblasts and mesenchymal stem cells and denominated by dermal equivalent (DE). Results show that it is possible to use the photomodulation process to control the wound healing in a scratching process and to induce the biomolecules release, both of which are related with the inflammatory wound healing process. In the studies, the MMP-2 and MMP-9 expression from zymography analyses were evaluated. All results showed a dependence on enzymatic activity relating to low-level laser applications which indicates a potential application in wound healing processes based on phototherapy and nanotechnology.
Keywords Dermal equivalents
mesenchymal stem cells
nanoemulsions
nanotechnology
phthalocyanine
tissue engineering
photodynamic process
human fibroblast
low-level laser therapy
matrix metalloproteinases
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2009/15363-9
FAPESP: 2008/53719-4
FAPESP: 2007/55319-0
FAPESP: 2006/50562-1
Date 2011-08-01
Published in Current Medicinal Chemistry. Sharjah: Bentham Science Publ Ltd, v. 18, n. 22, p. 3376-3381, 2011.
ISSN 0929-8673 (Sherpa/Romeo, impact factor)
Publisher Bentham Science Publ Ltd
Extent 3376-3381
Origin http://dx.doi.org/10.2174/092986711796504745
Access rights Closed access
Type Review
Web of Science ID WOS:000294405600012
URI http://repositorio.unifesp.br/11600/43448

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