Vascular adventitia is a suitable compartment to transplant transduced vascular smooth muscle cells for ex vivo gene expression

Vascular adventitia is a suitable compartment to transplant transduced vascular smooth muscle cells for ex vivo gene expression

Author Braga, Patricia Cristina Baleeiro Beltrao Autor UNIFESP Google Scholar
Koh, Ivan Hong Jun Autor UNIFESP Google Scholar
Silva, MRR Google Scholar
Gutierrez, P. S. Google Scholar
Han, Sang Won Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Vascular smooth muscle cells (VSMC) are ideal for systemic gene therapy because of their proximity to blood vessels and they have demonstrated long-term exogenous gene expression in vivo. However, the procedure generally followed to seed the transduced VSMC onto arteries denuded of endothelial cells usually induces stenosis and thrombosis, with a consequent high risk for use in humans. We demonstrate here that the vascular adventitia is a suitable place to introduce transduced VSMC and to secrete therapeutic proteins into the blood stream by a simple procedure, avoiding postoperative vascular complications. Transduced VSMC, with the retroviral vectors carrying the human growth hormone gene (hGH), were seeded into the adventitia of the rat abdominal aorta by single injection of a cell suspension. Based on the hGH and anti-hGH production in serum and on histological analysis of the removed aortas, we demonstrated hGH production over the 2-month experimental period. None of the animals used in the experiment showed stenosis, thrombosis, or other vascular or visible physiological abnormalities.
Keywords smooth muscle cells
adventitia
retroviral vector
gene therapy
hGH
Language English
Date 2002-01-01
Published in Cell Transplantation. Elmsford: Cognizant Communication Corp, v. 11, n. 6, p. 583-592, 2002.
ISSN 0963-6897 (Sherpa/Romeo, impact factor)
Publisher Cognizant Communication Corp
Extent 583-592
Origin http://doi.org/10.3727/000000002783985486
Access rights Closed access
Type Article
Web of Science ID WOS:000178834200012
URI http://repositorio.unifesp.br/11600/43256

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