MUTATIONS IN THE QUINOLONE RESISTANCE-DETERMINING REGIONS OF GYRA AND PARC IN ENTEROBACTERIACEAE ISOLATES FROM BRAZIL

MUTATIONS IN THE QUINOLONE RESISTANCE-DETERMINING REGIONS OF GYRA AND PARC IN ENTEROBACTERIACEAE ISOLATES FROM BRAZIL

Author Minarini, Luciene Andrade da Rocha Autor UNIFESP Google Scholar
Darini, Ana Lucia C. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Mutations in the quinolone resistance-determining regions (QRDR) in chromosomal gyrA and parC genes and fluoroquinolone susceptibility profiles were investigated in quinolone-resistant Enterobacteriaceae isolated from community and hospitalized patients in the Brazilian Southeast region. A total of 112 nalidixic acid-resistant enterobacterial isolates collected from 2000 to 2005 were investigated for mutations in the topoisomerases genes gyrA and parC by amplifying and sequencing the QRDR regions. Susceptibility to fluoroquinolones was tested by the agar dilution method. Amongst the 112 enterobacterial isolates, 81 (72.3%) were resistant to ciprofloxacin and 5 (4.5%) showed reduced susceptibility. Twenty-six (23.2%) were susceptible to ciprofloxacin. Several alterations were detected in gyrA and parC genes. Escherichia coli isolates (47.7%) showed double mutations in the gyrA gene and a single one in the parC gene. Two unusual aminoacid substitutions are reported, an Asp87-Asn in a Citrobacter freundii isolate with reduced susceptibility to fluoroquinolones and a Glu84-Ala in one E. coli isolate. Only a parC gene mutation was found in fluoroquinolone-susceptible Enterobacter aerogenes. None of the isolates susceptible to ciprofloxacin presented mutations in topoisomerase genes. This comprehensive analysis of QRDRs in gyrA and parC genes, covering commonly isolated Enterobacteriaceae in Brazil is the largest reported up to now.
Keywords quinolone resistance-determining region
mutations
gyrA
parC
Enterobacteriaceae
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 2006/02320-9
Date 2012-10-01
Published in Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 43, n. 4, p. 1309-1314, 2012.
Publisher Soc Brasileira Microbiologia
Extent 1309-1314
Origin http://dx.doi.org/10.1590/S1517-83822012000400010
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000315286400010
SciELO ID S1517-83822012000400010 (statistics in SciELO)
URI http://repositorio.unifesp.br/11600/43137

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