Mini-Mu insertions in the tetracycline resistance determinant from Proteus mirabilis

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dc.contributor.author Magalhaes, Vanda Dolabela de [UNIFESP]
dc.contributor.author Castilho, Beatriz Amaral de [UNIFESP]
dc.date.accessioned 2018-06-15T16:24:00Z
dc.date.available 2018-06-15T16:24:00Z
dc.date.issued 1997-03-01
dc.identifier http://dx.doi.org/10.1590/S0100-879X1997000300009
dc.identifier.citation Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 30, n. 3, p. 363-367, 1997.
dc.identifier.issn 0100-879X
dc.identifier.uri http://repositorio.unifesp.br/11600/43117
dc.description.abstract The inducible tetracycline resistance determinant isolated from Proteus mirabilis cloned into the plasmid pACYC177 was mutagenized by insertion of a mini-Mu-lac phage in order to define the regions in the cloned sequences encoding the structural and regulatory proteins. Three different types of mutants were obtained: one lost the resistance phenotype and became Lac(+); another expressed the resistance at lower levels and constitutively; the third was still dependent on induction but showed a lower minimal inhibitory concentration. The mutant phenotypes and the locations of the insertions indicate that the determinant is composed of a repressor gene and a structural gene which are not transcribed divergently as are other known tetracycline determinants isolated from Gram-negative bacteria. en
dc.format.extent 363-367
dc.language.iso eng
dc.publisher Assoc Bras Divulg Cientifica
dc.relation.ispartof Brazilian Journal Of Medical And Biological Research
dc.rights Acesso aberto
dc.subject tetracycline en
dc.subject mutation en
dc.subject lacZ-fusion en
dc.subject Proteus mirabilis en
dc.subject bacteriophage Mu en
dc.title Mini-Mu insertions in the tetracycline resistance determinant from Proteus mirabilis en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation UNIV FED SAO PAULO,ESCOLA PAULISTA MED,DEPT MICROBIOL IMMUNOL & PARASITOL,BR-04023062 SAO PAULO,BRAZIL
dc.description.affiliationUnifesp UNIV FED SAO PAULO,ESCOLA PAULISTA MED,DEPT MICROBIOL IMMUNOL & PARASITOL,BR-04023062 SAO PAULO,BRAZIL
dc.identifier.file S0100-879X1997000300009.pdf
dc.identifier.scielo S0100-879X1997000300009
dc.identifier.doi 10.1590/S0100-879X1997000300009
dc.description.source Web of Science
dc.identifier.wos WOS:A1997WQ16600009



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