Mini-Mu insertions in the tetracycline resistance determinant from Proteus mirabilis

Mini-Mu insertions in the tetracycline resistance determinant from Proteus mirabilis

Author Magalhaes, Vanda Dolabela de Autor UNIFESP Google Scholar
Castilho, Beatriz Amaral de Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The inducible tetracycline resistance determinant isolated from Proteus mirabilis cloned into the plasmid pACYC177 was mutagenized by insertion of a mini-Mu-lac phage in order to define the regions in the cloned sequences encoding the structural and regulatory proteins. Three different types of mutants were obtained: one lost the resistance phenotype and became Lac(+); another expressed the resistance at lower levels and constitutively; the third was still dependent on induction but showed a lower minimal inhibitory concentration. The mutant phenotypes and the locations of the insertions indicate that the determinant is composed of a repressor gene and a structural gene which are not transcribed divergently as are other known tetracycline determinants isolated from Gram-negative bacteria.
Keywords tetracycline
mutation
lacZ-fusion
Proteus mirabilis
bacteriophage Mu
Language English
Date 1997-03-01
Published in Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 30, n. 3, p. 363-367, 1997.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Assoc Bras Divulg Cientifica
Extent 363-367
Origin http://dx.doi.org/10.1590/S0100-879X1997000300009
Access rights Open access Open Access
Type Article
Web of Science ID WOS:A1997WQ16600009
SciELO ID S0100-879X1997000300009 (statistics in SciELO)
URI http://repositorio.unifesp.br/11600/43117

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