INHIBITION OF AMINOPEPTIDASE ACTIVITY BY AROMATIC AND OTHER CYCLIC-COMPOUNDS

INHIBITION OF AMINOPEPTIDASE ACTIVITY BY AROMATIC AND OTHER CYCLIC-COMPOUNDS

Author Alves, Kaethy Bisan Autor UNIFESP Google Scholar
Costa, R. H. Google Scholar
Aguilar, MAG Google Scholar
Freitas, J. O. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The effect of 2-naphthylamine, p-nitroaniline, o-phenanthroline, sodium deoxycholate and hydrocortisone succinate on the activity of human urine aminopeptidase, rat kidney methionyl and arginyl aminopeptidase, soybean and Enterolobium contortisiliquum seed aminopeptidase was studied using aminoacyl-2-naphthylamide and L-Leu-p-nitroanilide as substrates. Ki values ranged from 10 muM to 2.7 mM. On the basis of Ki and Km values, and catalytic efficiency for each enzyme, it is clear that the aminopeptidases from human urine and from soybean seed should be assayed with both substrates, whereas L-Leu-p-nitroaniline is a more appropriate substrate for the rat kidney aminopeptidases. Sodium deoxycholate is a better inhibitor than hydrocortisone succinate. Non-competitive inhibition was observed in all cases except for E. contortisiliquun seed aminopeptidase.
Keywords AMINOPEPTIDASE
ARYLAMIDASE
ENZYME INHIBITION
Language English
Date 1992-01-01
Published in Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 25, n. 11, p. 1103-1106, 1992.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Assoc Bras Divulg Cientifica
Extent 1103-1106
Access rights Closed access
Type Article
Web of Science ID WOS:A1992KD75800005
URI http://repositorio.unifesp.br/11600/43014

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