ANDROGEN REGULATION OF THE NICOTINIC ACETYLCHOLINE RECEPTOR-IONIC CHANNEL IN A HORMONE-DEPENDENT SKELETAL-MUSCLE

ANDROGEN REGULATION OF THE NICOTINIC ACETYLCHOLINE RECEPTOR-IONIC CHANNEL IN A HORMONE-DEPENDENT SKELETAL-MUSCLE

Author Souccar, Caden Autor UNIFESP Google Scholar
Yamamoto, Lucia Akie Autor UNIFESP Google Scholar
Goncalo, M. C. Google Scholar
Lapa, Antonio José Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The trophic influence of testosterone on the nicotinic acetylcholine receptor-ionic channel (AChR) was studied in the levator ani (LA) muscle of adult male rats (120 days) intact (C) or gonadectomized when 90 days old (G). In the indirectly elicited muscle twitch, the LA from G rats was less sensitive to d-tubocurarine (0.1-1-mu-M) than control muscles (IC25: C = 0.30-mu-M, G = 0.46-mu-M). In G rats, the amplitude of neurally evoked endplate currents (EPC) was reduced by 70%, but the EPC time constant was not changed. Maximal junctional binding of [I-125]alpha-bungarotoxin in the LA (C: 72.5 +/- 13.2 amol/endplate) was reduced by 1.8-fold in LA from G rats, with no change of the association rate constant (C: 5.64 +/- 1.29 10(6) M-1 min-1). The results indicate that testosterone deprivation reduces the junctional AChR density in the rat LA without modifying the binding properties of the receptor.
Keywords LEVATOR ANI MUSCLE
TESTOSTERONE
ACETYLCHOLINE RECEPTOR
END-PLATE CURRENTS
D-TUBOCURARINE
ALPHA-BUNGAROTOXIN
Language English
Date 1991-01-01
Published in Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 24, n. 10, p. 1051-1054, 1991.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Assoc Bras Divulg Cientifica
Extent 1051-1054
Access rights Closed access
Type Article
Web of Science ID WOS:A1991GR20300010
URI http://repositorio.unifesp.br/11600/42996

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