Amifostine Increases FAS and Caspase-3 Expression in Colonic Tissue of Irradiated Mice

Amifostine Increases FAS and Caspase-3 Expression in Colonic Tissue of Irradiated Mice

Author Oshima, Celina Tizuko Fujiyama Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Gomes, Thiago Simao Autor UNIFESP Google Scholar
Adios, Priscila C. Autor UNIFESP Google Scholar
Egami, Mizue Imoto Autor UNIFESP Google Scholar
Segreto, Helena Regina Comodo Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Background/Aim: The aim of this study was to evaluate the expression of FASL, FAS and FADD and caspase-3 in oesophagus, stomach and colonic tissues of mice irradiated in vivo by immunohistochemistry. Materials and Methods: A total of 48 adult male C57BL mice were distributed into four groups: Ami(-)/Rad(-): Mice received 0.5 ml of 0.9% physiological saline solution (PPS) intraperitioneally (i.p.); Ami(+)/Rad(-): mice received amifostine (400mg/kg i.p.) freshly dissolved in double-distilled water; Ami(-)/Rad(+): mice received 0.5 ml of PSS i.p. 30 min before a single whole-body radiation dose of 7 Gy; Ami(+)/Rad(+): mice received 0.5 ml of an aqueous solution of 400 mg/kg amifostine i.p.30 min prior to irradiation. All groups were assigned into subgroups sacrificed at 0.5 h, 1 h, 2 h and 4 h after irradiation. Results. In oesophagus and stomach tissues, we did not observe any difference between Ami(-)/ad(-), Ami(+)/Rad(-), Ami(-)/Rad(+) and Ami(+)/Rad(+) groups in the expression of FASL, FAS and FADD. The colonic tissue was the only to exhibit any difference in the expression of FAS and caspase-3 protein in the Ami(-)/Rad(+) group at 1 and 2 h. Amifostine increased FAS and caspase-3 immunoexpression when compared to the control. Immunoexpression for FASL and FADD was not remarkably different in colonic tissue. Conclusion: Taken together, our results demonstrate that amifostine increases FAS and caspase-3 expression in colonic tissue of irradiated mice.
Keywords Amifostine
Language English
Date 2015-05-01
Published in Anticancer Research. Athens: Int Inst Anticancer Research, v. 35, n. 5, p. 2817-2822, 2015.
ISSN 0250-7005 (Sherpa/Romeo, impact factor)
Publisher Int Inst Anticancer Research
Extent 2817-2822
Access rights Closed access
Type Article
Web of Science ID WOS:000354267200041

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