The use of nested Polymerase Chain Reaction (nested PCR) for the early diagnosis of Histoplasma capsulatum infection in serum and whole blood of HIV-positive patients

The use of nested Polymerase Chain Reaction (nested PCR) for the early diagnosis of Histoplasma capsulatum infection in serum and whole blood of HIV-positive patients

Alternative title Uso da Reação em Cadeia da Polimerase aninhada (PCR aninhada) para o diagnóstico precoce da infecção pelo Histoplasma capsulatum no soro e no sangue total de pacientes HIV positivos
Author Dantas, Katia Cristina Google Scholar
Freitas, Roseli S. Google Scholar
Moreira, Adriana Pardini Vicentini Autor UNIFESP Google Scholar
Silva, Marcos Vinicius da Google Scholar
Benard, Gil Google Scholar
Vasconcellos, Cidia Google Scholar
Criado, Paulo Ricardo Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
State Hlth Secretariat
Emilio Ribas Infectol Inst
Insitute State Publ Employees IAMSPE
Abstract The aim of the study was to detect the rDNA sequences and their regions in Histoplasma capsulatum, which could be considered species-specific and used as a molecular method for this diagnosis by the technique of nested polymerase chain reaction (nested PCR), employing specific sequences (primers) for H. capsulatum: 18S rDNA region (HC18), 100 kDa (HC100) and the sequence 5.8 S-ITS rDNA (HC5.8). The PCR sequences HC18, HC100 and HC5.8 resulted in a specificity of 100%. The molecular assays may increase the specificity, sensitivity and speed in the diagnosis of Histoplasmosis.
Keywords AIDS-related opportunistic infections
Histoplasma
HIV seropositivity
Molecular diagnostic techniques
Polymerase chain reaction
Language English
Sponsor Faculty of Medicine of the University of Sao Paulo (FMUSP)
Date 2013-01-01
Published in Anais Brasileiros De Dermatologia. Rio De Janeiro Rj: Soc Brasileira Dermatologia, v. 88, n. 1, p. 141-143, 2013.
ISSN 0365-0596 (Sherpa/Romeo, impact factor)
Publisher Soc Brasileira Dermatologia
Extent 141-143
Origin http://dx.doi.org/10.1590/S0365-05962013000100025
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000316807000025
SciELO ID S0365-05962013000100025 (statistics in SciELO)
URI http://repositorio.unifesp.br/11600/42508

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