A Na(+)-sensitive cation channel modulated by angiotensin II in cultured intestinal myocytes

A Na(+)-sensitive cation channel modulated by angiotensin II in cultured intestinal myocytes

Author Nouailhetas, Viviane Louise Andree Autor UNIFESP Google Scholar
Aboulafia, Jeannine Autor UNIFESP Google Scholar
Frediani-Neto, E. Autor UNIFESP Google Scholar
Ferreira, Alice Teixeira Autor UNIFESP Google Scholar
Paiva, Antonio Cechelli de Mattos Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Single-channel currents were recorded in excised inside-out and cell-attached patches of cultured cells from the longitudinal smooth muscle of the guinea pig ileum. In the presence of symmetrical high-K+ solutions, we identified a voltage-dependent 12-pS channel. It was reversibly blocked by addition of either Ba2+ or Cs+ at the cellular side of the patch but was insensitive to Ca2+ or ATP. This channel had poor selectivity concerning cations (P-Li > P-K = P-Na = P-Ca, where P is permeability) and low permeability to anions. Isosmotic substitution of NaCl for KCl in the solution facing the cellular side enhanced the channel activity by increasing NPo values where N is number of channels and P-o is open probability. In the cell-attached configuration, the channel was also activated by addition of angiotensin II in the bath solution. We propose that this nonselective cation channel might play a role in the control of the membrane potential during the contractile response of the guinea pig ileum to agonists by keeping the voltage-sensitive Ca2+ channels open.
Keywords GUINEA PIG ILEUM
NONSELECTIVE CATION CHANNEL
PATCH CLAMP
SODIUM MODULATION
Language English
Date 1994-06-01
Published in American Journal Of Physiology-cell Physiology. Bethesda: Amer Physiological Soc, v. 266, n. 6, p. C1538-C1543, 1994.
ISSN 0002-9513 (Sherpa/Romeo, impact factor)
Publisher Amer Physiological Soc
Extent C1538-C1543
Origin http://ajpcell.physiology.org/content/266/6/C1538
Access rights Closed access
Type Article
Web of Science ID WOS:A1994NV80900005
URI http://repositorio.unifesp.br/11600/42361

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account