Cytomegalovirus antigenemia in acquired immunodeficiency syndrome patients with untreated cytomegalovirus retinitis

Cytomegalovirus antigenemia in acquired immunodeficiency syndrome patients with untreated cytomegalovirus retinitis

Author Pannuti, Cláudio Sérgio Autor UNIFESP Google Scholar
Kallas, Esper Georges Autor UNIFESP Google Scholar
Muccioli, Cristina Autor UNIFESP Google Scholar
Roland, Ronald Kleinsorge Autor UNIFESP Google Scholar
Ferreira, Elizabeth C. Google Scholar
Bueno, Stella MHS Google Scholar
Canto, Cynhia L. Motta do Autor UNIFESP Google Scholar
Villas Boas, Lucy S. Google Scholar
Belfort, Rubens Junior Autor UNIFESP Google Scholar
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract PURPOSE: To determine the frequency of cytomegalovirus (CMV) viremia in patients with acquired immunodeficiency syndrome (AIDS) and untreated CMV retinitis using conventional cell culture isolation and the sensitive CMV antigenemia assay.METHODS: We examined 24 AIDS patients with ophthalmologic diagnosis of untreated CMV retinitis and 24 AIDS patients without present or past retinitis (control patients) from three medical centers between September 1992 and March 1994. Cytomegalovirus antigenemia was detected by an indirect peroxidase staining in 300,000 cytocentrifuged neutrophils, using a mixture of murine monoclonal antibodies directed against the pp65 lower matrix protein of CMV.RESULTS: Positive antigenemia was demonstrated in eight (33.3%) of the 24 retinitis patients and in none of the 24 control patients (P <.001). Only two of the eight antigenemia-positive patients had a concurrent positive CMV isolation from blood leukocytes by conventional cell culture assay.CONCLUSIONS: These results emphasize the risk of extraocular disease in AIDS patients with CMV retinitis because the virus is often present in peripheral blood leukocytes. The CMV antigenemia assay may be a simple and rapid means of identifying those patients with unilateral retinitis at highest risk of developing CMV retinitis of the fellow eye or of visceral CMV disease if intravitreal injections or implants are used as sole treatment for CMV retinitis.
Language English
Date 1996-12-01
Published in American Journal Of Ophthalmology. Chicago: Ophthalmic Publ Co, v. 122, n. 6, p. 847-852, 1996.
ISSN 0002-9394 (Sherpa/Romeo, impact factor)
Publisher Ophthalmic Publ Co
Extent 847-852
Access rights Closed access
Type Article
Web of Science ID WOS:A1996VW75800010

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