Effects of repeated extracorporeal shock wave on kidney apoptosis of normal and diabetic rat

Effects of repeated extracorporeal shock wave on kidney apoptosis of normal and diabetic rat

Author Kira, Vicente Massaji Autor UNIFESP Google Scholar
Fagundes, Djalma José Autor UNIFESP Google Scholar
Bandeira, César Orlando Peralta Autor UNIFESP Google Scholar
Kaufman, Oskar Autor UNIFESP Google Scholar
Fagundes, Anna T. N. Autor UNIFESP Google Scholar
Ortiz, Valdemar Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract OBJECTIVE: To assess the effect of repeated extracorporeal shock waves (ESW) on the apoptosis of renal parenchyma in normal and diabetic rats by the caspase-3 expression. MATERIALS AND METHODS: 80 rats were assigned for ESW (Direx Tripter X1® - 14 KVA) to one of three groups with 50% rats with diabetes (A) and 50% normal (B) in each: G1 (n = 40): no ESW; G2 (n = 20): one ESW; G3 (n = 20): two ESWin an interval of 14 days. The animals were sacrificed 3 days after the ESW and samples of the renal parenchyma were histologically prepared, stained by the caspase-3 cleavage immunohistochemical method, and the apoptotic index (ApIn) was calculated by computer program Image Pro-Plus V 4,5,1 Media Cybernetics® ( ApIn = number of apoptotic cells in the microscopic section / total cells in the microscopic section). RESULTS: There was no statistical difference in ApIn of normal rats with one ESW (8.4 + 0.2) or two ESW (9.1 + 0.3) and both were similar to the control group with no ESW (7.9 + 0.2). The ApIn of diabetic rats with one ESW (7.4 + 0.3) was similar to the normal rats with one or two ESW. On the other hand, the ApIn was statistically higher in diabetic rats with two ESW (11.9 + 0.4) than in diabetic rats with one ESW (7.4 + 0.3). CONCLUSIONS: The ApIn of normal rats with a single (2,000 SW) or repeated ESW (4,000 SW) was similar to the control animals without any ESW. In diabetic rats, the repeated ESW (4,000 SW) may result in an additional risk of tissue damage expressed by the higher ApIn.
Keywords kidney
lithotripsy
high-energy shock waves
diabetes
rats
apoptosis
Language English
Date 2008-02-01
Published in International braz j urol. Sociedade Brasileira de Urologia, v. 34, n. 1, p. 91-96, 2008.
ISSN 1677-5538 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira de Urologia
Extent 91-96
Origin http://dx.doi.org/10.1590/S1677-55382008000100013
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000207684600024
SciELO ID S1677-55382008000100013 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/4221

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