DNA and bone structure preservation in medieval human skeletons

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dc.contributor.author Coulson-Thomas, Yvette May [UNIFESP]
dc.contributor.author Norton, Andrew L.
dc.contributor.author Coulson-Thomas, Vivien Jane [UNIFESP]
dc.contributor.author Florencio-Silva, Rinaldo [UNIFESP]
dc.contributor.author Ali, Nadir
dc.contributor.author Elmrghni, Samir
dc.contributor.author Gil, Cristiane D. [UNIFESP]
dc.contributor.author Sasso, Gisela R. S. [UNIFESP]
dc.contributor.author Dixon, Ronald A.
dc.contributor.author Nader, Helena B. [UNIFESP]
dc.date.accessioned 2016-01-24T14:40:33Z
dc.date.available 2016-01-24T14:40:33Z
dc.date.issued 2015-06-01
dc.identifier http://dx.doi.org/10.1016/j.forsciint.2015.04.005
dc.identifier.citation Forensic Science International. Clare: Elsevier B.V., v. 251, p. 186-194, 2015.
dc.identifier.issn 0379-0738
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/39126
dc.description.abstract Morphological and ultrastructural data from archaeological human bones are scarce, particularly data that have been correlated with information on the preservation of molecules such as DNA. Here we examine the bone structure of macroscopically well-preserved medieval human skeletons by transmission electron microscopy and immunohistochemistry, and the quantity and quality of DNA extracted from these skeletons. DNA technology has been increasingly used for analyzing physical evidence in archaeological forensics; however, the isolation of ancient DNA is difficult since it is highly degraded, extraction yields are low and the co-extraction of PCR inhibitors is a problem. We adapted and optimised a method that is frequently used for isolating DNA from modern samples, Chelex (R) 100 (BioRad) extraction, for isolating DNA from archaeological human bones and teeth. the isolated DNA was analysed by real-time PCR using primers targeting the sex determining region on the Y chromosome (SRY) and STR typing using the AmpFlSTR (R) Identifiler PCR Amplification kit. Our results clearly show the preservation of bone matrix in medieval bones and the presence of intact osteocytes with well preserved encapsulated nuclei. in addition, we show how effective Chelex1 100 is for isolating ancient DNA from archaeological bones and teeth. This optimised method is suitable for STR typing using kits aimed specifically at degraded and difficult DNA templates since amplicons of up to 250 bp were successfully amplified. (C) 2015 Elsevier Ireland Ltd. All rights reserved. en
dc.format.extent 186-194
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Forensic Science International
dc.rights Acesso restrito
dc.subject Ancient DNA en
dc.subject Archaeological bone en
dc.subject Archaeological teeth en
dc.subject Chelex en
dc.subject DNA typing en
dc.subject Electron microscopy en
dc.title DNA and bone structure preservation in medieval human skeletons en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Lincoln Univ
dc.contributor.institution Univ Durham
dc.contributor.institution Univ Cambridge
dc.description.affiliation Universidade Federal de São Paulo, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.description.affiliation Lincoln Univ, Sch Life Sci, Lincoln LN6 7TS, England
dc.description.affiliation Univ Durham, Durham DH7 9RH, England
dc.description.affiliation Univ Cambridge, John van Geest Ctr Brain Repair, Cambridge CB2 0PY, England
dc.description.affiliation Universidade Federal de São Paulo, Dept Morphol & Genet, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biochem, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Morphol & Genet, BR-04023900 São Paulo, Brazil
dc.identifier.doi 10.1016/j.forsciint.2015.04.005
dc.description.source Web of Science
dc.identifier.wos WOS:000354125900028



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