Impairment of male reproductive function after sleep deprivation

Impairment of male reproductive function after sleep deprivation

Author Alvarenga, Tathiana A. Autor UNIFESP Google Scholar
Hirotsu, Camila Autor UNIFESP Google Scholar
Mazaro-Costa, Renata Google Scholar
Tufik, Sergio Autor UNIFESP Google Scholar
Andersen, Monica L. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Goiás (UFG)
Abstract Objective: To evaluate the influence of sleep loss on sexual behavior, hormone levels, sperm parameters, and testis-specific gene expression in male rats.Design: Experimental research.Setting: Animal laboratory.Animal(s): Male adult Wistar-Hannover rats.Intervention(s): Sexually experienced rats were subjected to paradoxic sleep deprivation (PSD) for 96 hours or sleep restriction (SR) for 21 days or kept in their home cage as control (CTRL).Main Outcome Measure(s): Sexual behavior, hormone levels, sperm parameters and expression of stress and nitric oxide-related genes were evaluated.Result(s): PSD significantly decreased sexual behavior compared with the CTRL group, whereas SR had no effect. the PSD group had significantly lower testosterone levels than the CTRL group. Both PSD and SR groups had lower sperm viabilities than the CTRL group. the decrease in the number of live sperm compared with the CTRL group was larger in the PSD group than in the SR group. Regarding testicular gene expression, both PSD and SR led to an increase of iNOS and hydroxysteroid 11 beta-dehydrogenase 1 expressions compared with the CTRL group. These changes were more pronounced in the PSD group. A significant increase in endothelial nitric oxide synthase expression was observed in the PSD groups compared with the CTRL group. No changes were observed in dimethylarginine dimethylaminohydrolase 1 and casein kinase 2 beta-polypeptide expressions.Conclusion(s): Sleep loss can promote marked changes in the male reproductive system of rats, particularly affecting spermatic function in part by interfering in the testicular nitric oxide pathway. (C) 2015 by American Society for Reproductive Medicine.
Keywords Sleep restriction
sexual behavior
testosterone
progesterone
sperm
reproduction
nitric oxide
male rat
iNOS
eNOS
Language English
Sponsor Associacao Fundo de Apoio a Pesquisa (AFIP)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
MEC-SeSU (PET) fellowship
Grant number FAPESP: 2014/15259-2
FAPESP: 11/12325-6
FAPESP: 12/05396-7
Date 2015-05-01
Published in Fertility and Sterility. New York: Elsevier B.V., v. 103, n. 5, p. 1355-+, 2015.
ISSN 0015-0282 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1355-+
Origin http://dx.doi.org/10.1016/j.fertnstert.2015.02.002
Access rights Closed access
Type Article
Web of Science ID WOS:000353843700042
URI http://repositorio.unifesp.br/handle/11600/39046

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