RTG1-and RTG2-dependent retrograde signaling controls mitochondrial activity and stress resistance in Saccharomyces cerevisiae

RTG1-and RTG2-dependent retrograde signaling controls mitochondrial activity and stress resistance in Saccharomyces cerevisiae

Author Torelli, Nicole Quesada Google Scholar
Ferreira-Junior, Jose Ribamar Google Scholar
Kowaltowski, Alicia J. Google Scholar
Cunha, Fernanda Marques da Autor UNIFESP Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Mitochondrial retrograde signaling is a communication pathway between the mitochondrion and the nucleus that regulates the expression of a subset of nuclear genes that codify mitochondrial proteins, mediating cell response to mitochondrial dysfunction. in Saccharomyces cerevisiae, the pathway depends on Rtg1p and Rtg3p, which together form the transcription factor that regulates gene expression, and Rtg2p, an activator of the pathway. Here, we provide novel studies aimed at assessing the functional impact of the lack of RTG-dependent signaling on mitochondrial activity. We show that mutants defective in RTG-dependent retrograde signaling present higher oxygen consumption and reduced hydrogen peroxide release in the stationary phase compared to wild-type cells. Interestingly, RTG mutants are less able to decompose hydrogen peroxide or maintain viability when challenged with hydrogen peroxide. Overall, our results indicate that RTG signaling is involved in the hormetic induction of antioxidant defenses and stress resistance. (C) 2015 Elsevier Inc. All rights reserved.
Keywords Retrograde signaling
Hormesis
Mitochondria
H2O2
Free radicals
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Pro-Reitoria de Pesquisa da Universidade de São Paulo (PRPUSP)
Grant number FAPESP: 2012/50500-7
FAPESP: 2010/519016
CNPq: 471162/2012-4
FAPESP: 2013/07937-8
Date 2015-04-01
Published in Free Radical Biology and Medicine. New York: Elsevier B.V., v. 81, p. 30-37, 2015.
ISSN 0891-5849 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 30-37
Origin http://dx.doi.org/10.1016/j.freeradbiomed.2014.12.025
Access rights Closed access
Type Article
Web of Science ID WOS:000352175800004
URI http://repositorio.unifesp.br/handle/11600/38949

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