Biocompatibility of the bacterial cellulose hydrogel in subcutaneous tissue of rabbits

Biocompatibility of the bacterial cellulose hydrogel in subcutaneous tissue of rabbits

Author Castro Pita, Pedro Celso de Google Scholar
Pinto, Flavia Cristina Morone Google Scholar
Lira, Mariana Montenegro de Melo Google Scholar
Melo, Francisco de Assis Dutra Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Aguiar, Jose Lamartine de Andrade Google Scholar
Institution Universidade Federal de Pernambuco (UFPE)
Univ Fed Rural Pernambuco
Universidade Federal de São Paulo (UNIFESP)
Abstract PURPOSE: To evaluate the biocompatibility and local sensibility reaction to bacterial cellulose hydrogel (0.8%) implanted in subcutaneous tissue of rabbits.METHODS: Fifteen New Zeland rabbits were randomly allocated into three groups: T-1, 7 days, T-2, 21 days, and T-3, 84 days. the new material was implanted in the subcutaneous tissue of the ear; on the scalp over the periosteum; and on the outer and inner surfaces of the thighs, in the aponeurosis of the muscle. At 7, 21 and 84 postoperative days, the material was collected for histological study. the clinical signs, inflammatory response, angiogenesis and fibrogenesis were variables used for analysis of the biocompatibility and biological reactivity to BCH. Analyses were performed with an AXIO (R) Imager. the statistical tests were performed using the GraphPad Prism 5.0 program (R)RESULTS: the intensity of the inflammatory infiltrate, considering the different cell types (PMN, LMN and GC), was statistically significant, with group T1 different from groups T2 and T3 (p = 0.0124 and p < 0.0001, respectively) and T2 different from the T3 group (p = 0.0007). Fibrogenesis grade 1 was the most prevalent in groups T1 (55.4%) and T2 (44.6%). the formation of neovascularization in the group was identified in 84.4% of samples.CONCLUSION: Bacterial cellulose hydrogel (0.8%) is biocompatible, integrating with the subcutaneous tissue of rabbits and inducing tissue remodeling.
Keywords Cellulose
Materials Testing
Bulking Agents
Subcutaneous Tissue
Language English
Sponsor Federal Government (Ministry of Science and Technology, MCT): FINEP (Studies and Projects Financing Agency)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2015-04-01
Published in Acta Cirurgica Brasileira. São Paulo: Acta Cirurgica Brasileira, v. 30, n. 4, p. 296-300, 2015.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Acta Cirurgica Brasileira
Extent 296-300
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000353700300009
SciELO ID S0102-865020150040000009 (statistics in SciELO)

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