Action of hyperbaric oxygenation in the rat skin flap

Action of hyperbaric oxygenation in the rat skin flap

Author Rech, Fabricio Valandro Autor UNIFESP Google Scholar
Fagundes, Anna Luiza Negrini Autor UNIFESP Google Scholar
Simoes, Ricardo Santos Autor UNIFESP Google Scholar
Florencio-Silva, Rinaldo Autor UNIFESP Google Scholar
Silva Sasso, Gisela Rodrigues da Autor UNIFESP Google Scholar
Taha, Murched Omar Autor UNIFESP Google Scholar
Fagundes, Djalma José Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract PURPOSE: To evaluate the morphology, necrotic area and collagen content in skin flaps of rats subjected to hyperbaric oxygenation (HBO).METHODS: Forty adult rats were divided into four groups: GEC - epilated; GE/HBO - epilated subjected to HBO; GER - epilated submitted to skin flap (2 cm in width /8 cm length in the dorsal area) and GER/HBO - epilated, subjected to skin flap and HBO. HBO (2.4 ATA) was performed for two hours during seven consecutive days. in the eighth day, the rats were anesthetized and the skin flaps were removed and separated into three portions, relative to pedicle fixation. the material fixed in 10% formalin was processed for paraffin embedding; sections were stained by H.E and subjected to picrosirius-red method. the slides examined under light microscopy for evaluation of the collagen content in polarized light microscope and ImageLab (R) software (Bio-Rad).RESULTS: the data showed larger area of necrosis and lower levels of collagen in the three regions of the GER group, whereas in the GER/HBO group the collagen content was similar to the GEC and GE/HBO groups.CONCLUSION: Hyperbaric oxygenation reduced the area of necrosis and preserved the morphology and collagen content in skin flaps of rats.
Keywords Skin
Hyperbaric Oxygenation
Collagen
Wound Healing
Rats
Language English
Date 2015-04-01
Published in Acta Cirurgica Brasileira. São Paulo: Acta Cirurgica Brasileira, v. 30, n. 4, p. 235-241, 2015.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Acta Cirurgica Brasileira
Extent 235-241
Origin http://dx.doi.org/10.1590/S0102-865020150040000001
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000353700300001
SciELO ID S0102-865020150040000001 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/38919

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