Dynamic changes in the spatio-temporal expression of the beta-defensin SPAG11C in the developing rat epididymis and its regulation by androgens

Dynamic changes in the spatio-temporal expression of the beta-defensin SPAG11C in the developing rat epididymis and its regulation by androgens

Author Ribeiro, Camilla M. Autor UNIFESP Google Scholar
Queiroz, Daniel B. C. Autor UNIFESP Google Scholar
Patrao, Marilia T. C. C. Autor UNIFESP Google Scholar
Denadai-Souza, Alexandre Autor UNIFESP Google Scholar
Romano, Renata M. Autor UNIFESP Google Scholar
Silva, Erick J. R. Autor UNIFESP Google Scholar
Avellar, Maria Christina W. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Herein, we characterized the spatio-temporal expression, cellular distribution and regulation by androgens of the P-defensin SPAG11C, the rat ortholog of the human SPAG11B isoform C, in the developing epididymis by using RT-PCR, in situ hybridization and immunohistochemistry. We observed that Spag11c mRNA was ubiquitously expressed in rat fetuses, but preferentially detected in male reproductive tissues at adulthood. SPAG11C (mRNA and protein) was prenatally mainly detected in the mesenchyme of the Wolffian duct, switching gradually after birth to a predominant localization in the epididymis epithelium during postnatal development. in the adult epididymis, smooth muscle and interstitial cells were also identified as sources of SPAG11C. Furthermore, SPAG11C was differentially immunolocalized on spermatozoa surface during their transit from testis throughout caput and cauda epididymis. Developmental and surgical castration studies suggested that androgens contribute to the epididymal cell type- and region-specific modulation of SPAG11C mRNA levels and immunolocalization. Together our findings provide novel insights into the potential role of beta-defensins in the epididymis. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
Keywords beta-Defensins
Male fertility
Epididymis
SPAG11B
Androgens
Development
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fogarty International Center Program for Training and Research in Population and Health
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fogarty International Center Program
Grant number FAPESP: 2010/52711-0
FAPESP: 2009/14649-3
CNPq: 480452/2008-3
Fogarty International Center Program for Training and Research in Population and Health: USA D43TW/HD00627
Fogarty International Center Program for Training and Research in Population and Health: UNIFESP/UNC 5-53284
CNPq: 401718/2012-3
CAPES: 02921/09-0
Date 2015-03-15
Published in Molecular and Cellular Endocrinology. Clare: Elsevier B.V., v. 404, n. C, p. 141-150, 2015.
ISSN 0303-7207 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 141-150
Origin http://dx.doi.org/10.1016/j.mce.2015.01.013
Access rights Closed access
Type Article
Web of Science ID WOS:000351645700016
URI http://repositorio.unifesp.br/handle/11600/38889

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