Adult exposure to bisphenol A (BPA) in Wistar rats reduces sperm quality with disruption of the hypothalamic-pituitary-testicular axis

Adult exposure to bisphenol A (BPA) in Wistar rats reduces sperm quality with disruption of the hypothalamic-pituitary-testicular axis

Author Wisniewski, Patricia Google Scholar
Romano, Renata M. Google Scholar
Kizys, Marina M. L. Autor UNIFESP Google Scholar
Oliveira, Kelen C. Autor UNIFESP Google Scholar
Kasamatsu, Teresa Autor UNIFESP Google Scholar
Giannocco, Gisele Autor UNIFESP Google Scholar
Chiamolera, Maria I. Autor UNIFESP Google Scholar
Dias-da-Silva, Magnus R. Autor UNIFESP Google Scholar
Romano, Marco A. Google Scholar
Institution State Univ Ctr Ooeste
Universidade Federal de São Paulo (UNIFESP)
Abstract Reproductive physiology involves complex biological processes that can be disrupted by exposure to environmental contaminants. the effects of bisphenol A (BPA) on spermatogenesis and sperm quality is still unclear. the objective of this study was to investigate the reproductive toxicity of BPA at dosages considered to be safe (5 or 25 mg BPA/kg/day). We assessed multiple sperm parameters, the relative expression of genes involved in the central regulation of the hypothalamic-pituitary-testicular axis, and the serum concentrations of testosterone, estradiol, LH and FSH. BPA exposure reduced sperm production, reserves and transit time. Significant damage to the acrosomes and the plasma membrane with reduced mitochondrial activity and increased levels of defective spermatozoa may have compromised sperm function and caused faster movement through the epididymis. BPA exposure reduced the serum concentrations of testosterone, LH and FSH and increased the concentration of estradiol. the relative gene expression revealed an increase in gonadotropin releasing hormone receptor (Gnrhr), luteinizing hormone beta (Lhb), follicle stimulating hormone beta (Fshb), estrogen receptor beta (Esr2) and androgen receptor (Ar) transcripts in the pituitary and a reduction in estrogen receptor alpha (Esr1) transcripts in the hypothalamus. in this study, we demonstrated for the first time that adult male exposure to BPA caused a reduction in sperm production and specific functional parameters. the corresponding pattern of gene expression is indicative of an attempt by the pituitary to reestablish normal levels of LH, FSH and testosterone serum concentrations. in conclusion, these data suggest that at dosages previously considered nontoxic to reproductive function, BPA compromises the spermatozoa and disrupts the hypothalamic-pituitary-gonadal axis, causing a state of hypogonadotropic hypogonadism. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
Keywords Bisphenol A
Sperm function
Sperm production
Gene expression
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number CAPES: 23038.009865/2013-32
Date 2015-03-02
Published in Toxicology. Clare: Elsevier B.V., v. 329, p. 1-9, 2015.
ISSN 0300-483X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1-9
Access rights Closed access
Type Article
Web of Science ID WOS:000350519500001

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