Chemotactic signal transduction and phosphate metabolism as adaptive strategies during citrus canker induction by Xanthomonas citri

Chemotactic signal transduction and phosphate metabolism as adaptive strategies during citrus canker induction by Xanthomonas citri

Author Moreira, Leandro Marcio Google Scholar
Facincani, Agda Paula Google Scholar
Ferreira, Cristiano Barbalho Google Scholar
Ferreira, Rafael Marine Google Scholar
Tiraboshi Ferro, Maria Ines Google Scholar
Gozzo, Fabio Cesar Google Scholar
Franco de Oliveira, Julio Cezar Autor UNIFESP Google Scholar
Ferro, Jesus Aparecido Google Scholar
Soares, Marcia Regina Google Scholar
Institution Univ Fed Ouro Preto
UNESP Univ Estadual Paulista
Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do Rio de Janeiro (UFRJ)
Abstract The genome of Xanthomonas citri subsp. Citri strain 306 pathotype A (Xac) was completely sequenced more than 10 years; to date, few studies involving functional genomics Xac and its host compatible have been developed, specially related to adaptive events that allow the survival of Xac within the plant. Proteomic analysis of Xac showed that the processes of chemotactic signal transduction and phosphate metabolism are key adaptive strategies during the interaction of a pathogenic bacterium with its plant host. the results also indicate the importance of a group of proteins that may not be directly related to the classical virulence factors, but that are likely fundamental to the success of the initial stages of the infection, such as methyl-accepting chemotaxis protein (Mcp) and phosphate specific transport (Pst). Furthermore, the analysis of the mutant of the gene pstB which codifies to an ABC phosphate transporter subunit revealed a complete absence of citrus canker symptoms when inoculated in compatible hosts. We also conducted an in silico analysis which established the possible network of genes regulated by two-component systems PhoPQ and PhoBR (related to phosphate metabolism), and possible transcriptional factor binding site (TFBS) motifs of regulatory proteins PhoB and PhoP, detaching high degree of conservation of PhoB TFBS in 84 genes of Xac genome. This is the first time that chemotaxis signal transduction and phosphate metabolism were therefore indicated to be fundamental to the process of colonization of plant tissue during the induction of disease associated with Xanthomonas genus bacteria.
Keywords Xanthomonas adaptation
Chemotaxis signal transduction
Phosphate metabolism
Two-component systems PhoBR and PhoPQ
Virulence
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
Grant number FAPESP: 04/02006-7
FAPEMIG: FAPEMIG-CBB-APQ-04425-10
Date 2015-03-01
Published in Functional & Integrative Genomics. Heidelberg: Springer Heidelberg, v. 15, n. 2, p. 197-210, 2015.
ISSN 1438-793X (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 197-210
Origin http://dx.doi.org/10.1007/s10142-014-0414-z
Access rights Closed access
Type Article
Web of Science ID WOS:000351397700007
URI http://repositorio.unifesp.br/handle/11600/38813

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