Differences in neonatal exposure to estradiol or testosterone on ovarian function and hormonal levels

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dc.contributor.author Marcondes, Rodrigo R.
dc.contributor.author Carvalho, Katia C.
dc.contributor.author Duarte, Daniele C.
dc.contributor.author Garcia, Natalia
dc.contributor.author Amaral, Vinicius C.
dc.contributor.author Simoes, Manuel J. [UNIFESP]
dc.contributor.author Lo Turco, Edson G. [UNIFESP]
dc.contributor.author Soares, Jose M.
dc.contributor.author Baracat, Edmund C.
dc.contributor.author Maciel, Gustavo A. R.
dc.date.accessioned 2016-01-24T14:40:00Z
dc.date.available 2016-01-24T14:40:00Z
dc.date.issued 2015-02-01
dc.identifier http://dx.doi.org/10.1016/j.ygcen.2015.01.006
dc.identifier.citation General and Comparative Endocrinology. San Diego: Academic Press Inc Elsevier Science, v. 212, p. 28-33, 2015.
dc.identifier.issn 0016-6480
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/38708
dc.description.abstract Exposure to an excess of androgen or estrogen can induce changes in reproductive function in adult animals that resemble polycystic ovary syndrome in humans. However, considerable differences exist among several types of animal models. Little is known about the molecular features of steroidogenesis and folliculogenesis in the ovaries of rats exposed to different sex steroids as neonates. Here, we evaluated the impact of androgen and estrogen exposure on the ovaries of adult female rats during their neonatal period in the gene expression of Lhr and Cyp17a1, two key players of steroidogenesis. We also assessed hormone levels, folliculogenesis and the theca-interstitial cell population. the study was performed on the second postnatal day in thirty female Wistar rats that were sorted into the following three intervention groups: testosterone, estradiol and vehicle (control group). the animals were euthanized 90 days after birth. the main outcomes were hormone serum levels, ovary histomorphometry and gene expression of Lhr and Cyp17a1 as analyzed via quantitative real-time PCR. We found that exposure to excess testosterone in early life increased the LH and testosterone serum levels, the LH/FSH ratio, ovarian theca-interstitial area and gene expression of Lhr and Cyp17a1 in adult rats. Estrogen induced an increase in the ovarian theca-interstitial area, the secondary follicle population and gene expression of Lhr and Cyp17a1. All animals exposed to the sex steroids presented with closed vaginas. Our data suggest that testosterone resulted in more pronounced reproductive changes than did estrogen exposure. Our results might provide some insight into the role of different hormones on reproductive development and on the heterogeneity of clinical manifestations of conditions such as polycystic ovary syndrome. (C) 2015 Elsevier Inc. All rights reserved. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 28-33
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof General and Comparative Endocrinology
dc.rights Acesso restrito
dc.subject Polycystic ovary syndrome en
dc.subject Animal models en
dc.subject LH receptor en
dc.subject Steroid en
dc.subject 17-Alpha-hydroxylase en
dc.title Differences in neonatal exposure to estradiol or testosterone on ovarian function and hormonal levels en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ São Paulo, Fac Med, Disciplina Ginecol, Lab Ginecol Estrutural & Mol LIM 58, BR-01246903 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Morfol & Genet, Disciplina Histol & Biol Estrutural, BR-04023900 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Cirurgia, Disciplina Urol, Setor Reprod Humana, BR-04024002 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Morfol & Genet, Disciplina Histol & Biol Estrutural, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Cirurgia, Disciplina Urol, Setor Reprod Humana, BR-04024002 São Paulo, Brazil
dc.description.sponsorshipID FAPESP: 2010/17417-3
dc.description.sponsorshipID CNPq: 134694/2010-4
dc.identifier.doi 10.1016/j.ygcen.2015.01.006
dc.description.source Web of Science
dc.identifier.wos WOS:000352176000004



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