Follicular fluid lipid fingerprinting from women with PCOS and hyper response during IVF treatment

Follicular fluid lipid fingerprinting from women with PCOS and hyper response during IVF treatment

Author Cordeiro, Fernanda Bertuccez Autor UNIFESP Google Scholar
Cataldi, Thais Regiani Autor UNIFESP Google Scholar
Teixeira da Costa, Livia do Vale Autor UNIFESP Google Scholar
Lima, Camila Bruna de Autor UNIFESP Google Scholar
Stevanato, Juliana Autor UNIFESP Google Scholar
Zylbersztejn, Daniel Suslik Autor UNIFESP Google Scholar
Ferreira, Christina Ramires Google Scholar
Eberlin, Marcos Nogueira Google Scholar
Cedenho, Agnaldo Pereira Autor UNIFESP Google Scholar
Lo Turco, Edson Guimaraes Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Abstract Polycystic ovary syndrome (PCOS) is an endocrine-metabolic disorder that leads to lower natural reproductive potential and presents a challenge for assisted reproductive medicine because patients may exhibit immature oocyte retrieval and a higher risk of ovarian hyper stimulation syndrome during in vitro fertilization (IVF) treatment. This study aimed to identify potential lipid biomarkers for women with PCOS and a hyper response to controlled ovarian stimulation.Follicular fluid samples were collected from patients who underwent IVF, including normal responder women who became pregnant (control group, n = 11), women with PCOS and a hyper response to gonadotropins (PCOS group, n = 7) and women with only hyper response to gonadotropins (HR group, n = 7). A lipidomic analysis was performed by electrospray ionization mass spectrometry, and candidate biomarkers were analyzed by tandem mass spectrometry experiment.The lipid profiles indicated particularities related to differences in phosphatidylcholine (PCOS and HR), phosphatidylserine, phosphatydilinositol and phosphatidylglycerol (control), sphingolipids (PCOS) and phosphatidylethanolamine (control and HR).These findings contribute to the understanding of the molecular mechanisms associated with lipid metabolism in the PCOS-related hyper response, and strongly suggest that these lipids may be useful as biomarkers, leading to the development of more individualized treatment for pregnancy outcome.
Keywords Polycystic ovary syndrome
Hyper response
Follicular fluid
Lipids
ESI-MS
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2012/06389-4
Date 2015-01-01
Published in Journal of Assisted Reproduction and Genetics. New York: Springer/plenum Publishers, v. 32, n. 1, p. 45-54, 2015.
ISSN 1058-0468 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 45-54
Origin http://dx.doi.org/10.1007/s10815-014-0375-0
Access rights Closed access
Type Article
Web of Science ID WOS:000347949800006
URI http://repositorio.unifesp.br/handle/11600/38575

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