Expression profile of neurotransmitter receptor and regulatory genes in the prefrontal cortex of spontaneously hypertensive rats: Relevance to neuropsychiatric disorders

Expression profile of neurotransmitter receptor and regulatory genes in the prefrontal cortex of spontaneously hypertensive rats: Relevance to neuropsychiatric disorders

Author Santoro, Marcos Leite Autor UNIFESP Google Scholar
Santos, Camila Mauricio Autor UNIFESP Google Scholar
Ota, Vanessa Kiyomi Autor UNIFESP Google Scholar
Gadelha, Ary Autor UNIFESP Google Scholar
Stilhano, Roberta Sessa Autor UNIFESP Google Scholar
Diana, Mariana Cepollaro Autor UNIFESP Google Scholar
Silva, Patricia Natalia Autor UNIFESP Google Scholar
Nery Spindola, Leticia Maria Autor UNIFESP Google Scholar
Melaragno, Maria Isabel Autor UNIFESP Google Scholar
Bressan, Rodrigo Affonseca Autor UNIFESP Google Scholar
Han, Sang Won Autor UNIFESP Google Scholar
Abilio, Vanessa Costhek Autor UNIFESP Google Scholar
Belangero, Sintia Iole Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Interdisciplinmy Lab Clin Neurosci LiNC
Abstract The spontaneously hypertensive rat (SHR) strain was shown to be a useful animal model to study several behavioral, pathophysiological and pharmacological aspects of schizophrenia and attention-deficit/hyperactivity disorder. To further understand the genetic underpinnings of this model, our primary goal in this study was to compare the gene expression profile of neurotransmitter receptors and regulators in the prefrontal cortex (PFC) and nucleus accumbens (NAcc) of SHR and Wistar rats (control group). in addition, we investigated DNA methylation pattern of promoter region of the genes differentially expressed. We performed gene expression analysis using a PCRarray technology, which simultaneously measures the expression of 84 genes related to neurotransmission. Four genes were significantly downregulated in the PFC of SHR compared to Wistar rats (Gad2, Chrnb4, Slc5a7, and Qrfpr) and none in nucleus accumbens. Gad2 and Qrfpr have CpG islands in their promoter region. for both, the promoter region was hypomethylated in SHR group, and probably this mechanism is not related with the downregulation of these genes. in summary, we identified genes that are downregulated in the PFC of SHR, and might be related to the behavioral abnormalities exhibited by this strain. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
Keywords Spontaneously hypertensive rats
Gene expression
DNA methylation
Gad2
Qrfpr
Slc5a7
Chrnb4
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2014-11-30
Published in Psychiatry Research. Clare: Elsevier B.V., v. 219, n. 3, p. 674-679, 2014.
ISSN 0165-1781 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 674-679
Origin http://dx.doi.org/10.1016/j.psychres.2014.05.034
Access rights Closed access
Type Article
Web of Science ID WOS:000341901600041
URI http://repositorio.unifesp.br/handle/11600/38471

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