Antimutagenic and antigenotoxic potential of grape juice concentrate in blood and liver of rats exposed to cadmium

Antimutagenic and antigenotoxic potential of grape juice concentrate in blood and liver of rats exposed to cadmium

Author Gomes de Moura, Carolina Foot Autor UNIFESP Google Scholar
Pidone Ribeiro, Flavia Andressa Autor UNIFESP Google Scholar
Pacheco de Jesus, Gustavo Protasio Autor UNIFESP Google Scholar
Pereira da Silva, Victor Hugo Autor UNIFESP Google Scholar
Oshima, Celina Tizuko Fujiyama Autor UNIFESP Google Scholar
Boiago Golluecke, Andrea Pittelli Autor UNIFESP Google Scholar
Aguiar, Odair Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The aim of this study was to evaluate the antimutagenic and antigenotoxic potential of grape juice concentrate in rodent organs exposed to cadmium chloride intoxication. A total of 15 Wistar rats were distributed into three groups (n=5), as follows: control group (CTRL; nontreated group), cadmium group (Cd), and cadmium-grape juice group (Cd+GJ). Exposed animals received intraperitoneal injection of cadmium chloride (1.2 mg/kg body weight) diluted in water and, after 15 days, Cd+GJ group received grape juice concentrate for 15 days, by gavage (0.8 mL, 1.18 mg of polyphenols kg(-1) day(-1)). Grape juice concentrate was able to decrease genotoxic effects induced by cadmium in peripheral blood and liver cells as depicted by single cell gel (comet) and micronucleus assays. A decrease for anti-8-hydroxy-20-deoxyguanosine (8OHdG) expression in hepatocytes of animals exposed to cadmium and treated with grape juice concentrate was also detected. Higher CuZn-SOD activity was observed in liver cells of the Cd+GJ group. No remarkable differences were seen regarding Mn-SOD activity among groups. Taken together, our results demonstrate that grape juice concentrate was able to exert antimutagenic and antigenotoxic activities in blood and liver cells of rats exposed to cadmium.
Keywords Grape juice
Cadmium
Genotoxicity
Mutagenicity
Oxidative stress
Rat
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2014-11-01
Published in Environmental Science and Pollution Research. Heidelberg: Springer Heidelberg, v. 21, n. 22, p. 13118-13126, 2014.
ISSN 0944-1344 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 13118-13126
Origin http://dx.doi.org/10.1007/s11356-014-3257-1
Access rights Closed access
Type Article
Web of Science ID WOS:000344546900047
URI http://repositorio.unifesp.br/handle/11600/38381

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