Impact of adrenalectomy and dexamethasone treatment on testicular morphology and sperm parameters in rats: insights into the adrenal control of male reproduction

Impact of adrenalectomy and dexamethasone treatment on testicular morphology and sperm parameters in rats: insights into the adrenal control of male reproduction

Author Silva, E. J. R. Autor UNIFESP Google Scholar
Vendramini, V. Autor UNIFESP Google Scholar
Restelli, A. Autor UNIFESP Google Scholar
Bertolla, R. P. Autor UNIFESP Google Scholar
Kempinas, W. G. Google Scholar
Avellar, M. C. W. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Here we investigated the hypothesis that normal levels of glucocorticoids, a class of adrenal steroid hormones, are required for normal testicular and epididymal functions. We examined the effects of the manipulation of glucocorticoid plasma levels by bilateral adrenalectomy (1, 2, 7 and 15days) alone or in combination with daily treatment with the synthetic glucocorticoid dexamethasone (DEX; 5g/kg, i.p., 6days) on the morphology of the testis and sperm parameters in rats. We showed that adrenalectomy led to a reduction in testicular sperm count and daily sperm production starting 2days after surgery and a differential decrease in sperm count in the epididymis, according to the region and time post-adrenalectomy analysed. in parallel, testes from 7-day adrenalectomized (ADX) rats displayed a higher frequency of damaged seminiferous tubules and the presence of elongated spermatids retained in the basal epithelial compartment in stages IX-XVII, which is indicative of defective spermiation. the alkaline comet assay revealed a late effect of adrenalectomy on epididymal sperm DNA fragmentation, which was increased only 15days after surgery. DEX treatment prevented the changes in testicular and epididymal sperm count observed in 7-day ADX rats, but failed to protect the testis from ADX-induced morphological abnormalities. Thus, our results indicated that glucocorticoids may be involved in events related to the maintenance of spermatogenesis and sperm maturation during adulthood. These findings provide new insights into the importance of adrenal steroids to male fertility.
Keywords adrenalectomy
animal models
epididymis
hormones
rat
sperm quality parameters
testis
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Fogarty International Center Program for Training and Research in Population and Health, USA
Universidade Federal de São Paulo/University of North Carolina at Chapel Hill
Grant number Fogarty International Center Program for Training and Research in Population and Health, USA: D43TW/HD00627
Date 2014-11-01
Published in Andrology. Hoboken: Wiley-Blackwell, v. 2, n. 6, p. 835-846, 2014.
ISSN 2047-2919 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 835-846
Origin http://dx.doi.org/10.1111/j.2047-2927.2014.00228.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000344328700005
URI http://repositorio.unifesp.br/handle/11600/38365

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