MMP-9 Expression Increases According to the Grade of Squamous Intraepithelial Lesion in Cervical Smears

MMP-9 Expression Increases According to the Grade of Squamous Intraepithelial Lesion in Cervical Smears

Author Matheus, Erika R. Google Scholar
Zonta, Marco A. Google Scholar
Discacciati, Michelle G. Google Scholar
Paruci, Priscila Google Scholar
Velame, Fernanda Google Scholar
Cardeal, Laura B. S. Google Scholar
Barros, Silvia B. M. Google Scholar
Pignatari, Antonio C. Autor UNIFESP Google Scholar
Maria-Engler, Silvya S. Google Scholar
Institution Universidade de São Paulo (USP)
In Cito Lab Diagnost Cytol
Universidade Federal de São Paulo (UNIFESP)
Abstract Studies about cervical carcinogenesis have demonstrated the increased expression of matrix-metalloproteinase (MMP) according to the grade of cervical intraepithelial lesions. Considering the importance of innovative techniques to introduce noninvasive and rapid diagnoses for patients, this study aimed to perform MMP-9 immunocytochemistry in cervical smears according to the cytopathological diagnoses, in order to monitor MMP activity in cervical smears. This cross-sectional study investigated the expression of MMP-9 in normal cervical smears, inflammatory cervical smears, squamous intraepithelial lesions, and cervical carcinoma. Cervical smears from 630 women were collected for cytopathological diagnoses and immunocytochemistry. Women with squamous intraepithelial lesions showed an increase in MMP-9 expression, with moderate to intense staining occurring with increasing cervical lesion grade. the prevalence of moderate to intense MMP-9 staining was 9% in normal cervical smears, 12% in cervical inflammation, 24% in low-grade squamous intraepithelial lesion (LSIL), 92% in high-grade squamous intraepithelial lesions (HSIL) and 100% in cervical carcinoma cases. in the specific case of LSIL, we found that association with MMP-9 is more evident when there is the simultaneous presence of an infectious agent. Thus, the expression of MMP-9 in cervical smears increases according to the grade of cervical lesion and LSIL in the presence of infectious agents showed higher MMP-9 expression than women with LSIL without infectious agents. Diagn. Cytopathol. 2014;42:827-833. (c) 2014 Wiley Periodicals, Inc.
Keywords matrix metalloproteinase 9
cervical smear
cervical cancer
human papillomavirus
cervical intraepithelial neoplasia
genital tract infections
uterine cervical cancer
pap smear
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Pro-Reitoria de Pos-Graduacao da Universidade de São Paulo (PRPG-USP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 2008/58817-4
FAPESP: 2012/09746-2
INCT_if: 573663/2008-4
Date 2014-10-01
Published in Diagnostic Cytopathology. Hoboken: Wiley-Blackwell, v. 42, n. 10, p. 827-833, 2014.
ISSN 8755-1039 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 827-833
Access rights Closed access
Type Article
Web of Science ID WOS:000342690200001

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