Gene Expression Analysis in Patients With Traumatic Anterior Shoulder Instability Suggests Deregulation of Collagen Genes

Gene Expression Analysis in Patients With Traumatic Anterior Shoulder Instability Suggests Deregulation of Collagen Genes

Author Belangero, Paulo Santoro Autor UNIFESP Google Scholar
Leal, Mariana Ferreira Autor UNIFESP Google Scholar
Figueiredo, Eduardo Antonio Autor UNIFESP Google Scholar
Cohen, Carina Autor UNIFESP Google Scholar
Pochini, Alberto de Castro Autor UNIFESP Google Scholar
Smith, Marilia Cardoso Autor UNIFESP Google Scholar
Andreoli, Carlos Vicente Autor UNIFESP Google Scholar
Belangero, Sintia Iole Autor UNIFESP Google Scholar
Ejnisman, Benno Autor UNIFESP Google Scholar
Cohen, Moises Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Shoulder dislocation occurs in 1-2% of the population. Capsular deformation is a key factor in shoulder dislocation; however, little is known about capsule biology. We evaluated, for the first time in literature, the expression of COL1A1, COL1A2, COL3A1 and COL5A1 in the antero-inferior, antero-superior and posterior regions of the glenohumeral capsule of 31 patients with anterior shoulder instability and eight controls. the expression of collagen genes was evaluated by quantitative reverse transcription-PCR. the expression of COL1A1, COL3A1 and the ratio of COL1A1/COL1A2 were increased in all three portions of the capsule in patients compared to controls (p<0.05). COL1A2 expression was upregulated in the antero-superior and posterior sites of the capsule of patients (p<0.05). the ratio of COL1A2/COL3A1 expression was reduced in capsule antero-inferior and posterior sites of patients compared to controls (p<0.05). in the capsule antero-inferior site of patients, the ratios of COL1A1/COL5A1, CO1A2/COL5A1 and COL3A1/COL5A1 expression were increased (p<0.05). in patients, COL1A1/COL5A1 was also increased in the posterior site (p<0.05). We found deregulated expression of collagen genes across the capsule of shoulder instability patients. These molecular alterations may lead to modifications of collagen fibril structure and impairment of the healing process, possibly with a role in capsular deformation. (C) 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
Keywords shoulder instability
glenohumeral capsule
gene expression
extracellular matrix
collagen
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2014-10-01
Published in Journal of Orthopaedic Research. Hoboken: Wiley-Blackwell, v. 32, n. 10, p. 1311-1316, 2014.
ISSN 0736-0266 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 1311-1316
Origin http://dx.doi.org/10.1002/jor.22680
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000340588000010
URI http://repositorio.unifesp.br/handle/11600/38233

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