Bothropoides insularis venom cytotoxicity in renal tubular epithelia cells

Bothropoides insularis venom cytotoxicity in renal tubular epithelia cells

Author Mello, Clarissa P. Google Scholar
Morais, Isabel C. O. Google Scholar
Menezes, Ramon R. P. P. B. Google Scholar
Pereira, Gustavo J. S. Autor UNIFESP Google Scholar
Torres, Alba F. C. Google Scholar
Lima, Danya B. Google Scholar
Pereira, Ticiana P. Google Scholar
Toyama, Marcos H. Google Scholar
Monteiro, Helena S. A. Google Scholar
Smaili, Soraya Soubhi Autor UNIFESP Google Scholar
Martins, Alice M. C. Google Scholar
Institution Univ Fed Ceara
Universidade Federal de São Paulo (UNIFESP)
Univ Estadual Paulista
Abstract Bothropoides insularis (jararaca-ilhoa) is a native endemic snake limited to the specific region of Queimada Island, on São Paulo coast. Several local and systemic effects have been described due to envenomation caused by it, such as edema, tissue necrosis, hemorrhage and acute renal failure. Our previous studies have shown that Bothropoides insularis venom (BinsV) demonstrated important functional and morphologic alterations in rat isolated kidney, especially decrease in tubular electrolyte transport, osmotic clearance and tubular necrosis. in order to elucidate the direct nephrotoxicity mechanism, the aim of the present study was to investigate BinsV cytotoxicity effect on renal epithelial cells. the treatment with BinsV over MDCK culture decreased cell viability in all concentrations tested with IC50 of 9 mu g/mL. BinsV was able to induce membrane rupture and cell death with phosphatidilserine externalization. Furthermore, BinsV induced ROS overproduction and mitochondrial membrane potential collapse, as well as Bax translocation and caspases 3 and 7 expression. Therefore, these events might be responsible by BinsV-induced cell death caused by mitochondrial dysfunction and ROS overproduction in the direct cytotoxicity process. (C) 2014 Elsevier B.V. All rights reserved.
Keywords Bothropoides insularis venom
Nephrotoxicity
Cell death
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Fundacao Cearense de Apoio ao Desenvolvimento Cientifico e Tecnologico do Estado do Ceara (FUNCAP)
Date 2014-09-15
Published in Toxicon. Oxford: Pergamon-Elsevier B.V., v. 88, p. 107-114, 2014.
ISSN 0041-0101 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 107-114
Origin http://dx.doi.org/10.1016/j.toxicon.2014.05.009
Access rights Closed access
Type Article
Web of Science ID WOS:000340691800013
URI http://repositorio.unifesp.br/handle/11600/38223

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