Hyaluronidase Alters the Lipid Profile of Cumulus Cells as Detected by MALDI-TOF MS and Multivariate Analysis

Hyaluronidase Alters the Lipid Profile of Cumulus Cells as Detected by MALDI-TOF MS and Multivariate Analysis

Author Montani, Daniela Antunes Autor UNIFESP Google Scholar
Regiani, Thais Autor UNIFESP Google Scholar
Victorino, Amanda Begati Autor UNIFESP Google Scholar
Camillo, Jacqueline Autor UNIFESP Google Scholar
Pilau, Eduardo Jorge Google Scholar
Gozzo, Fabio Cesar Google Scholar
Zylbersztejn, Daniel Suslik Autor UNIFESP Google Scholar
Ferreira, Christina Ramires Google Scholar
Lo Turco, Edson Guimaraes Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Abstract This research aimed to study the changes in lipid composition in cumulus cells using hyaluronidase according to the intracytoplasmic sperm injection protocol commonly used in human reproduction clinics. the lipid extraction was performed by the Blight-Dyer protocol and the lipid profiles were obtained by MALDI-TOF MS in positive and negative modes. the mass spectra data were processed with MassLynx and the statistical analysis was performed using MetaboAnalyst 2.0. Fifteen ions were selected for each mode as potential markers for differences between the groups. These ions were identified in the human metabolome database as phosphatidylserine with and without treatment, phosphatidylethanolamine in the after treatment group and phosphatidylinositol in the before treatment group, which are lipids that may be involved in cell apoptosis and signaling. We concluded that MALDI-TOF MS coupled with multivariate analysis can be utilized as a strategy to obtain and study the lipid profiles of cumulus cells and as a tool to study the metabolic state of cumulus cells.
Keywords Lipids
Lipid profile
Cumulus cells
Hyaluronidase
MALDI-TOF MS
Multivariate analysis
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2010/14,732-5
Date 2014-09-01
Published in Lipids. Heidelberg: Springer Heidelberg, v. 49, n. 9, p. 957-962, 2014.
ISSN 0024-4201 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 957-962
Origin http://dx.doi.org/10.1007/s11745-014-3931-6
Access rights Closed access
Type Article
Web of Science ID WOS:000341085800009
URI http://repositorio.unifesp.br/handle/11600/38144

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