Influence of ovarian hormones deprivation on gene expression in the lower urinary tract of rats

Influence of ovarian hormones deprivation on gene expression in the lower urinary tract of rats

Author Bortolini, Maria Augusta Tezelli Autor UNIFESP Google Scholar
Silva, Ismael Dale Cotrim Guerreiro da Autor UNIFESP Google Scholar
Hamerski, Maria G. Autor UNIFESP Google Scholar
Castro, Rodrigo de Aquino Autor UNIFESP Google Scholar
Sartori, Marair Gracio Ferreira Autor UNIFESP Google Scholar
Girão, Manoel João Batista Castello Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract OBJECTIVE: Identify the influence of ovarian hormone deprivation in expression genes on the lower urinary tract of rats. MATERIALS AND METHODS: This study deals with gene screening on lower urinary tract of rats. Fifty isogenic rats divided in two groups of twenty-five animals have their lower urinary tract surgically removed: group I, ovariectomized rats 30 days prior to surgery; group II, non-ovariectomized rats. Total RNA was isolated from bladder and urethra, and differential expression of genes was analyzed quantitative, qualitative and comparatively by array technology and RT-PCR. RESULTS: A total of 76 candidate genes were identified as differentially expressed between the groups, 26 being lower expressed in group II, and 50 in group I. Among them, differential expression validation was confirmed by RT-PCR for three lower expressed genes in group I: Vascular Endothelial Growth Factor (VEGF), Beta-2 Microglobulin (B2M) and Cytochrome c Oxidase subunit I (COX I). CONCLUSION: Ovarian hormone deprivation influences the expression genes on lower urinary tract. We demonstrated that a 30-day period of castration down regulate the expression of VEGF, B2M and COX I in adult rats which are involved in activities of angiogenesis, immune responses and cellular metabolism respectively.
Keywords ovary
urinary tract
microarray analysis
Language English
Date 2007-08-01
Published in International braz j urol. Sociedade Brasileira de Urologia, v. 33, n. 4, p. 544-553, 2007.
ISSN 1677-5538 (Sherpa/Romeo)
Publisher Sociedade Brasileira de Urologia
Extent 544-553
Access rights Open access Open Access
Type Article
SciELO ID S1677-55382007000400014 (statistics in SciELO)

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