BENEFICIAL INFLUENCE of PHYSICAL EXERCISE FOLLOWING STATUS EPILEPTICUS in the IMMATURE BRAIN of RATS

BENEFICIAL INFLUENCE of PHYSICAL EXERCISE FOLLOWING STATUS EPILEPTICUS in the IMMATURE BRAIN of RATS

Author Novaes Gomes, F. G. Autor UNIFESP Google Scholar
Da Silva, S. Gomes Autor UNIFESP Google Scholar
Cavalheiro, E. A. Autor UNIFESP Google Scholar
Arida, R. M. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Hosp Israelita Albert Einstein
Abstract Studies in adult animals have demonstrated a beneficial effect of physical exercise on epileptic insults. Although the effects of physical exercise on the mature nervous system are well documented, its influence on the developing nervous system subjected to injuries in childhood has been little explored. the purpose of our study was to investigate whether a physical exercise program applied during brain development could influence the hippocampal plasticity of rats submitted to status epilepticus (SE) induced by pilocarpine model at two different ages of the postnatal period. Male Wistar rats aged 18 (P18) and 28 (P28) days were randomly divided into four groups: Control (CTRL), Exercise (EX), SE (SE) and SE Exercise (SE/EX) (n = 17 per group). After the aerobic exercise program, histological and behavioral (water maze) analyses were performed. Our results showed that only animals subjected to pilocarpine-induced SE at P28 presented spontaneous seizures during the observational period. A significant reduction in seizure frequency was observed in the SE/EX group compared to the SE group. in adulthood, animals submitted to early-life SE displayed impairment in long-term memory in the water maze task, while the exercise program reversed this deficit. Reduced mossy fiber sprouting in the dentate gyrus was noted in animals that presented spontaneous seizures (SE/EX vs SE). Exercise increased cell proliferation (Ki-67 staining) and anti-apoptotic response (bcl-2 staining) and reduced pro-apoptotic response (Bax staining) in animals of both ages of SE induction (P18/28). Exercise also modified the brain-derived neurotrophic factor (BDNF) levels in EX and SE/EX animals. Our findings indicate that in animals subjected to SE in the postnatal period a physical exercise program brings about beneficial effects on seizure frequency and hippocampal plasticity in later stages of life. (C) 2014 IBRO. Published by Elsevier B.V. All rights reserved.
Keywords physical exercise
status epilepticus
immature brain
brain development
epilepsy
neuroplasticity
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
INNT, Brazil
Date 2014-08-22
Published in Neuroscience. Oxford: Pergamon-Elsevier B.V., v. 274, p. 69-81, 2014.
ISSN 0306-4522 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 69-81
Origin http://dx.doi.org/10.1016/j.neuroscience.2014.05.024
Access rights Closed access
Type Article
Web of Science ID WOS:000338712300008
URI http://repositorio.unifesp.br/handle/11600/38102

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account