Flavones from Erythrina falcata are modulators of fear memory

Flavones from Erythrina falcata are modulators of fear memory

Author Oliveira, Daniela Rodrigues de Autor UNIFESP Google Scholar
Zamberlam, Claudia Raquel Autor UNIFESP Google Scholar
Gaiardo, Renan Barreta Autor UNIFESP Google Scholar
Rego, Gizelda Maia Google Scholar
Cerutti, Janete Maria Autor UNIFESP Google Scholar
Cavalheiro, Alberto J. Google Scholar
Cerutti, Suzete Maria Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade de São Paulo (USP)
Abstract Background: Flavonoids, which have been identified in a variety of plants, have been demonstrated to elicit beneficial effects on memory. Some studies have reported that flavonoids derived from Erythrina plants can provide such beneficial effects on memory. the aim of this study was to identify the flavonoids present in the stem bark crude extract of Erythrina falcata (CE) and to perform a bioactivity-guided study on conditioned fear memory.Methods: the secondary metabolites of CE were identified by high performance liquid chromatography combined with a diode array detector, electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI/MSn) and nuclear magnetic resonance (NMR). the buthanolic fraction (BuF) was obtained by partitioning. Subfractions from BuF (BuF1 - BuF6) and fraction flavonoidic (FfA and FfB) were obtained by flash chromatography. the BuF3 and BuF4 fractions were used for the isolation of flavonoids, which was performed using HPLC-PAD. the isolated substances were quantified by HPLC-DAD and their structures were confirmed by nuclear magnetic resonance (NMR). the activities of CE and the subfractions were monitored using a one-trial, step-down inhibitory avoidance (IA) task to identify the effects of these substances on the acquisition and extinction of conditioned fear in rats.Results: Six subclasses of flavonoids were identified for the first time in CE. According to our behavioral data, CE, BuF, BuF3 and BuF4, the flavonoidic fractions, vitexin, isovitexin and 6-C-glycoside-diosmetin improved the acquisition of fear memory. Rats treated with BuF, BuF3 and BuF4 were particularly resistant to extinction. Nevertheless, rats treated with FfA and FfB, vitexin, isovitexin and 6-C-glycoside-diosmetin exhibited gradual reduction in conditioned fear response during the extinction retest session, which was measured at 48 to 480 h after conditioning.Conclusions: Our results demonstrate that vitexin, isovitexin and diosmetin-6-C-glucoside and flavonoidic fractions resulted in a significant retention of fear memory but did not prevent the extinction of fear memory. These results further substantiate that the treatment with pure flavonoids or flavanoid-rich fractions might represent potential therapeutic approaches for the treatment of neurocognitive disorders, improvement of memory acquisition and spontaneous recovery of fear.
Keywords Flavone
Acquisition
Extinction
Fear conditioning
Erythrina falcata (Fabaceae)
HPLC-ESI/MSn
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Brazilian Agricultural Research Corporation (EMBRAPA)
Grant number FAPESP: 2009/15229-8
Date 2014-08-05
Published in Bmc Complementary and Alternative Medicine. London: Biomed Central Ltd, v. 14, 17 p., 2014.
ISSN 1472-6882 (Sherpa/Romeo, impact factor)
Publisher Biomed Central Ltd
Extent 17
Origin http://dx.doi.org/10.1186/1472-6882-14-288
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000341109000003
URI http://repositorio.unifesp.br/handle/11600/38080

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