Chemopreventive activity of apple extract following medium-term oral carcinogenesis assay induced by 4-nitroquinoline-1-oxide

Chemopreventive activity of apple extract following medium-term oral carcinogenesis assay induced by 4-nitroquinoline-1-oxide

Author Pidone Ribeiro, Flavia Andressa Autor UNIFESP Google Scholar
Gomes de Moura, Carolina Foot Autor UNIFESP Google Scholar
Boiago Gollucke, Andrea Pitelli Google Scholar
Ferreira, Monica Siqueira Google Scholar
Catharino, Rodrigo Ramos Google Scholar
Aguiar, Odair Autor UNIFESP Google Scholar
Spadari, Regina Celia Autor UNIFESP Google Scholar
Barbisan, Luis Fernando Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Catholic Santos Univ
Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
Abstract Objective: the aim of this study was to evaluate the chemopreventive activity of an apple extract following medium-term oral carcinogenesis assay induced by 4-nitroquinoline-1-oxide (4NQO).Methods: A total of 30 male Wistar rats were distributed into five groups as follows (n = 6 per group): Group 1, negative control group (non-treated group); Group 2, received 4NQO during 8 weeks in drinking water and treated with apple extract at 1% by gavage between the first and fourth weeks daily (initiation phase); Group 3, received 4NQO for 8 weeks in drinking water and treated with apple extract by gavage at 1% between the fifth and eighth weeks daily (promotion phase); Group 4, received apple extract at 1% by gavage for 8 consecutive weeks only; and Group 5, received 4NQO for 8 weeks in drinking water daily.Results: Histopathological analysis revealed decreased hyperplasic lesions in Group 2 when compared with Group 5. Likewise, decreased dysplastic lesions in Group 3 were observed when compared with Group 5. in Groups 2 and 3, decreased COX-2 and TNF-alpha gene expressions were observed when compared with Group 5. Cytochrome c and caspase 3 levels increased in Groups 2 and 3 when compared with Group 5.Conclusion: in conclusion, our results demonstrate that apple extract suppresses rat tongue carcinogenesis as a result of anti-inflammatory activity and apoptosis through the intrinsic mitochondrial pathway. (C) 2014 Elsevier B.V. All rights reserved.
Keywords Apple extract
4-Nitroquinoline-1-oxide
Oral cancer
Inflammation
Apoptosis
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2014-08-01
Published in Archives of Oral Biology. Oxford: Pergamon-Elsevier B.V., v. 59, n. 8, p. 815-821, 2014.
ISSN 0003-9969 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 815-821
Origin http://dx.doi.org/10.1016/j.archoralbio.2014.04.013
Access rights Closed access
Type Article
Web of Science ID WOS:000338822300007
URI http://repositorio.unifesp.br/handle/11600/38040

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