Investigation of Apoptosis-Related Gene Expression Levels in Preimplantation Biopsies as Predictors of Delayed Kidney Graft Function

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dc.contributor.author Goncalves-Primo, Amador
dc.contributor.author Mourao, Tuila B. [UNIFESP]
dc.contributor.author Andrade-Oliveira, Vinicius [UNIFESP]
dc.contributor.author Campos, Erika F. [UNIFESP]
dc.contributor.author Medina-Pestana, Jose O. [UNIFESP]
dc.contributor.author Tedesco-Silva, Helio
dc.contributor.author Gerbase-DeLima, Maria [UNIFESP]
dc.date.accessioned 2016-01-24T14:37:28Z
dc.date.available 2016-01-24T14:37:28Z
dc.date.issued 2014-06-27
dc.identifier http://dx.doi.org/10.1097/01.TP.0000442579.12285.e8
dc.identifier.citation Transplantation. Philadelphia: Lippincott Williams & Wilkins, v. 97, n. 12, p. 1260-1265, 2014.
dc.identifier.issn 0041-1337
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/37882
dc.description.abstract Background the purpose of this study was to investigate the expression of the gene coding for the antiapoptotic molecule Bcl-2, the proapoptotic molecule Bax, and the apoptosis executor enzyme caspase-3 in preimplantation renal biopsies (PIB) as markers for delayed graft function.Methods in this prospective single-center study, gene expression levels were evaluated using real-time TaqMan polymerase chain reaction in PIB of kidneys from 72 deceased donors (DDs) and 18 living donors (LDs).ResultsCASP3 and BAX expression levels were higher, whereas those of BCL2 were lower, in DD than in LD PIB. in biopsies from DD, BCL2 levels were lower in cases with DGF, whereas no differences were observed concerning CASP3 and BAX. the BAX/BCL2 gene expression ratio greater than 2.29 associated with DGF with an odds ratio of 2.00. A multiple regression analysis including data of TLR4 expression in the first day posttransplant PB from a previous study of our group conducted in the same patients revealed a very strong association of the combination of BAX/BCL2 greater than 2.3 in PIB and TLR4 of 0.95 uRE or lesser in PB with the occurrence of DGF, with OR of 120 and positive and negative predictive values of 91% and 92%, respectively.Conclusions the power to predict DGF of the combination of high BAX/BCL2 expression in PIB and low TLR4 expression in the first day posttransplant peripheral blood observed in the present study is extremely high, in comparison to any other marker or combinations of markers so far published in the literature. en
dc.description.sponsorship Associacao Fundo de Incentivo a Pesquisa (AFIP)
dc.format.extent 1260-1265
dc.language.iso eng
dc.publisher Lippincott Williams & Wilkins
dc.relation.ispartof Transplantation
dc.rights Acesso restrito
dc.subject Kidney transplant en
dc.subject Preimplantation biopsies en
dc.subject Delayed graft function en
dc.subject Apoptosis en
dc.subject Gene expression en
dc.title Investigation of Apoptosis-Related Gene Expression Levels in Preimplantation Biopsies as Predictors of Delayed Kidney Graft Function en
dc.type Artigo
dc.contributor.institution Inst Imunogenet AFIP
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Hosp Rim & Hipertensao
dc.description.affiliation Inst Imunogenet AFIP, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, São Paulo, Brazil
dc.description.affiliation Hosp Rim & Hipertensao, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, São Paulo, Brazil
dc.identifier.doi 10.1097/01.TP.0000442579.12285.e8
dc.description.source Web of Science
dc.identifier.wos WOS:000337726300015



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