Investigation of Apoptosis-Related Gene Expression Levels in Preimplantation Biopsies as Predictors of Delayed Kidney Graft Function

Investigation of Apoptosis-Related Gene Expression Levels in Preimplantation Biopsies as Predictors of Delayed Kidney Graft Function

Author Goncalves-Primo, Amador Google Scholar
Mourao, Tuila B. Autor UNIFESP Google Scholar
Andrade-Oliveira, Vinicius Autor UNIFESP Google Scholar
Campos, Erika F. Autor UNIFESP Google Scholar
Medina-Pestana, Jose O. Autor UNIFESP Google Scholar
Tedesco-Silva, Helio Google Scholar
Gerbase-DeLima, Maria Autor UNIFESP Google Scholar
Institution Inst Imunogenet AFIP
Universidade Federal de São Paulo (UNIFESP)
Hosp Rim & Hipertensao
Abstract Background the purpose of this study was to investigate the expression of the gene coding for the antiapoptotic molecule Bcl-2, the proapoptotic molecule Bax, and the apoptosis executor enzyme caspase-3 in preimplantation renal biopsies (PIB) as markers for delayed graft function.Methods in this prospective single-center study, gene expression levels were evaluated using real-time TaqMan polymerase chain reaction in PIB of kidneys from 72 deceased donors (DDs) and 18 living donors (LDs).ResultsCASP3 and BAX expression levels were higher, whereas those of BCL2 were lower, in DD than in LD PIB. in biopsies from DD, BCL2 levels were lower in cases with DGF, whereas no differences were observed concerning CASP3 and BAX. the BAX/BCL2 gene expression ratio greater than 2.29 associated with DGF with an odds ratio of 2.00. A multiple regression analysis including data of TLR4 expression in the first day posttransplant PB from a previous study of our group conducted in the same patients revealed a very strong association of the combination of BAX/BCL2 greater than 2.3 in PIB and TLR4 of 0.95 uRE or lesser in PB with the occurrence of DGF, with OR of 120 and positive and negative predictive values of 91% and 92%, respectively.Conclusions the power to predict DGF of the combination of high BAX/BCL2 expression in PIB and low TLR4 expression in the first day posttransplant peripheral blood observed in the present study is extremely high, in comparison to any other marker or combinations of markers so far published in the literature.
Keywords Kidney transplant
Preimplantation biopsies
Delayed graft function
Gene expression
Language English
Sponsor Associacao Fundo de Incentivo a Pesquisa (AFIP)
Date 2014-06-27
Published in Transplantation. Philadelphia: Lippincott Williams & Wilkins, v. 97, n. 12, p. 1260-1265, 2014.
ISSN 0041-1337 (Sherpa/Romeo, impact factor)
Publisher Lippincott Williams & Wilkins
Extent 1260-1265
Access rights Closed access
Type Article
Web of Science ID WOS:000337726300015

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