Anti-tumor activity of grape juice concentrate in the rat tongue two-stage initiation-promotion protocol induced by 4-nitroquinoline 1-oxide

Anti-tumor activity of grape juice concentrate in the rat tongue two-stage initiation-promotion protocol induced by 4-nitroquinoline 1-oxide

Author Pacheco de Jesus, Gustavo Protasio Autor UNIFESP Google Scholar
Pidone Ribeiro, Flavia Andressa Autor UNIFESP Google Scholar
Gomes de Moura, Carolina Foot Autor UNIFESP Google Scholar
Boiago Gollucke, Andrea Pitelli Google Scholar
Oshima, Celina Tizuko Fujiyama Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Catholic Santos Univ
Abstract The aim of this study was to evaluate the anti-tumor activity of grape juice concentrate following medium-term oral carcinogenesis assay induced by 4-nitroquinoline 1-oxide (4NQO). A total of 30 male Wistar rats were distributed into five groups, as follows (n = 6 per group): Group 1 - negative control group (non-treated group); Group 2 - received grape juice concentrate at 1% dose by gavage for eight consecutive weeks; Group 3 - received 4NQO for 8 weeks at 20 ppm dose in drinking water daily; Group 4 - received 4NQO at 20 ppm dose during 8 weeks in drinking water and treated with grape juice concentrate at 1% dose orally by gavage for first 4 weeks after 4-NQO administration; Group 5 - received 4NQO at 20 ppm dose for 8 weeks in drinking water and treated with grape juice concentrate at 1% dose orally by gavage between the 5th and 8th weeks daily. Histopathological analysis revealed a decrease in hyperplasic and dysplastic lesions in Group 4. Groups 4 and 5 showed decreased COX-2 and TNF-alpha and eNOS gene expression. Grape juice concentrate also increased SOD Cu/Zn and catalase expression. However, Ki-67 immunoexpression was reduced at the promotion step of oral carcinogenesis (G5). Taken together, our results demonstrate that grape juice concentrate modulates rat tongue carcinogenesis as a result of anti-inflammatory activity, antioxidant activity and down-regulation of oral cells proliferation.
Keywords 4 nitroquinoline 1-oxide
chemoprevention
grape juice
oral cancer
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2014-05-01
Published in Toxicology Mechanisms and Methods. London: Informa Healthcare, v. 24, n. 4, p. 276-283, 2014.
ISSN 1537-6516 (Sherpa/Romeo, impact factor)
Publisher Informa Healthcare
Extent 276-283
Origin http://dx.doi.org/10.3109/15376516.2014.881944
Access rights Closed access
Type Article
Web of Science ID WOS:000335513200005
URI http://repositorio.unifesp.br/handle/11600/37763

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