The morphological and molecular characterization of Henneguya rotunda n. sp., a parasite of the gill arch and fins of Salminus brasiliensis from the Mogi Guacu River, Brazil

The morphological and molecular characterization of Henneguya rotunda n. sp., a parasite of the gill arch and fins of Salminus brasiliensis from the Mogi Guacu River, Brazil

Author Moreira, Gabriel Sassarão Alves Google Scholar
Adriano, Edson Aparecido Autor UNIFESP Google Scholar
Silva, Marcia Ramos Monteiro da Google Scholar
Ceccarelli, Paulo Sergio Google Scholar
Maia, Antonio Augusto Mendes Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Inst Chico Mendes Conservacao Biodiversidade ICMb
Abstract A new species of myxosporea (Henneguya rotunda n. sp.) was found in the membrane of the gill arch and the fins of Salminus brasiliensis in the Mogi Gua double dagger u River, municipality of Pirassununga, So Paulo state, Brazil. Morphological and morphometric analyses using light microscopy revealed parasites with similar characteristics at both infection sites. the mature spores found infecting the fins had oval spore body with 7.1 +/- 0.2 mu m in length, 5.6 +/- 0.2 mu m in width, 3.7 +/- 0.1 mu m in thickness, 16.4 +/- 1.2 mu m in length of the caudal process, and 23.6 +/- 1.1 mu m in total length of the spore. in a frontal view, the polar capsule was observed to be symmetrical with 3.4 +/- 0.2 mu m in length and 1.8 +/- 0.1 mu m in width. Mature spores contain six to seven turns of the polar filaments. the morphometric data concerning the spores obtained from plasmodia from the membrane of the gill arch were similar to those from the fins. Ultrastructure analysis revealed that the plasmodial wall was formed by a single membrane and had numerous pinocytotic canals connecting the outside of the plasmodia to the ectoplasm zone. Beyond that, various electron-translucent vesicles also were observed at the periphery of the plasmodium. the molecular analyses of the 18S rDNA gene from the spores obtained from the gill arch membrane and fin membrane showed that these sequences shared 100 % similarity. Phylogenetic studies using maximum parsimony and maximum likelihood methods demonstrated the polyphyletic clustering of the myxosporean parasites of characiform fishes. H. rotunda n. sp. clustered as a sister species of Myxobolus pantanalis, also a parasite of S. brasiliensis.
Keywords Natural environment
Salminus brasiliensis
Myxozoa
Ultrastructure
18S rDNA
Neotropics
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number CNPq: 477658/2010-5
Date 2014-05-01
Published in Parasitology Research. New York: Springer, v. 113, n. 5, p. 1703-1711, 2014.
ISSN 0932-0113 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 1703-1711
Origin http://dx.doi.org/10.1007/s00436-014-3815-y
Access rights Closed access
Type Article
Web of Science ID WOS:000335157200010
URI http://repositorio.unifesp.br/handle/11600/37713

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