Pyrostegia venusta heptane extract containing saturated aliphatic hydrocarbons induces apoptosis on B16F10-Nex2 melanoma cells and displays antitumor activity in vivo

Pyrostegia venusta heptane extract containing saturated aliphatic hydrocarbons induces apoptosis on B16F10-Nex2 melanoma cells and displays antitumor activity in vivo

Author Figueiredo, Carlos Rogerio Autor UNIFESP Google Scholar
Matsuo, Alisson Leonardo Autor UNIFESP Google Scholar
Pereira, Felipe Valença Autor UNIFESP Google Scholar
Rabaca, Aline Nogueira Autor UNIFESP Google Scholar
Farias, Camyla Fernandez de Autor UNIFESP Google Scholar
Girola, Natalia Autor UNIFESP Google Scholar
Massaoka, Mariana Hiromi Autor UNIFESP Google Scholar
Azevedo, Ricardo Alexandre de Autor UNIFESP Google Scholar
Scutti, Jorge Augusto Borin Autor UNIFESP Google Scholar
Arruda, Denise Costa Autor UNIFESP Google Scholar
Silva, Luciana Pereira Google Scholar
Rodrigues, Elaine Guadelupe Autor UNIFESP Google Scholar
Lago, Joao Henrique Ghilardi Autor UNIFESP Google Scholar
Travassos, Luiz Rodolpho Autor UNIFESP Google Scholar
Silva, Regildo Márico Gonçalves da Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Estadual Paulista UNESP
Abstract Background: Pyrostegia venusta (Ker. Gawl.) Miers (Bignoniacea) is a medicinal plant from the Brazilian Cerrado used to treat leucoderma and common diseases of the respiratory system. Objective: To investigate the antitumor activity of P.venusta extracts against melanoma. Materials and Methods: the cytotoxic activity and tumor induced cell death of heptane extract (HE) from P. venusta flowers was evaluated against murine melanoma B16F10-Nex2 cells in vitro and in a syngeneic model in vivo. Results: We found that HE induced apoptosis in melanoma cells by disruption of the mitochondrial membrane potential, induction of reactive oxygen species and late apoptosis evidenced by plasma membrane blebbing, cell shrinkage, chromatin condensation and DNA fragmentation, exposure of phosphatidylserine on the cell surface and activation of caspase-2,-3,-8,-9. HE was also protective against singeneyc subcutaneous melanoma HE compounds were also able to induce cell cycle arrest at G2/M phases on tumor cells. On fractionation of HE in silica gel we isolated a cytotoxic fraction that contained a mixture of saturated hydrocarbons identified by (1) H NMR and GC-MS analyses. Predominant species were octacosane (C 28 H 58 -36%) and triacontane (C 30 H 62 -13%), which individually showed significant cytotoxic activity against murine melanoma B16F10-Nex2 cells in vitro and a very promising antitumor protection against subcutaneous melanoma in vivo. Conclusion: the results suggest that the components of the heptane extract, mainly octasane and triacontane, which showed antitumor properties in experimental melanoma upon regional administration, might also be therapeutic in human cancer, such as in the mostly epidermal and slowly invasive melanomas, such as acral lentiginous melanoma, as an adjuvant treatment to surgical excision.
Keywords Apoptosis
cytotoxicity
melanoma
Pyrostegia venusta
saturated hydrocarbons
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2014-05-01
Published in Pharmacognosy Magazine. Mumbai: Medknow Publications & Media Pvt Ltd, v. 10, n. 38, p. 363-376, 2014.
ISSN 0973-1296 (Sherpa/Romeo, impact factor)
Publisher Medknow Publications & Media Pvt Ltd
Extent 363-376
Origin http://dx.doi.org/10.4103/0973-1296.133284
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000341787400024
URI http://repositorio.unifesp.br/handle/11600/37704

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