Impact of Angiogenic Therapy in the Treatment of Critical Lower Limb Ischemia in an Animal Model

Impact of Angiogenic Therapy in the Treatment of Critical Lower Limb Ischemia in an Animal Model

Author Ocke Reis, Paulo Eduardo Google Scholar
Carvalho, Leonardo Pinto de Autor UNIFESP Google Scholar
Yasumura, Eduardo Autor UNIFESP Google Scholar
Silva, Flavia Helena da Autor UNIFESP Google Scholar
Garcia, Bianca Cristina Autor UNIFESP Google Scholar
Beutel, Abram Autor UNIFESP Google Scholar
Sacramento, Chester Bittencourt Autor UNIFESP Google Scholar
Costa Baptista-Silva, Jose Carlos Autor UNIFESP Google Scholar
Campos, Ruy Ribeiro de Autor UNIFESP Google Scholar
Takiya, Christina Maeda Google Scholar
Borojevic, Radovan Google Scholar
Han, Sang Won Autor UNIFESP Google Scholar
Institution Universidade Federal Fluminense (UFF)
Procardiaco Hosp
Universidade Federal de São Paulo (UNIFESP)
Fed Univ Rio Grande do Sul UFRS
Universidade Federal do Rio de Janeiro (UFRJ)
Abstract Angiogenic therapies for critical limb ischemia were tested in a mouse model. the mice were anesthetized and their femoral arteries were ligated. the animals were treated with bone marrow mononuclear cells (BMMCs) alone, BMMCs combined with plasmid vector encoding granulocyte macrophage colony-stimulating factor (GM-CSF), received no treatment, or no intervention (controls). the degree of ischemia was monitored for 4 weeks using a visual scale. Muscle atrophy and strength were assessed at 4 weeks postoperatively; the mice were then killed. in treated animals, total necrosis of the limb was not found, the weight of the gastrocnemius and quadriceps muscles was significantly higher, functional ability and tissue regeneration were significantly increased, and muscle impairment and adipocyte presence were significantly reduced compared with untreated animals. At inducing angiogenesis, the BMMCs alone was more effective than BMMCs combined with plasmid vector encoding GM-CSF. Treated animals showed increased angiogenesis compared with ischemic untreated ones.
Keywords angiogenesis-inducing agents
gene therapy
cell therapy
ischemia
lower extremity
Language English
Date 2014-04-01
Published in Vascular and Endovascular Surgery. Thousand Oaks: Sage Publications Inc, v. 48, n. 3, p. 207-216, 2014.
ISSN 1538-5744 (Sherpa/Romeo, impact factor)
Publisher Sage Publications Inc
Extent 207-216
Origin http://dx.doi.org/10.1177/1538574413518119
Access rights Closed access
Type Article
Web of Science ID WOS:000332270100003
URI http://repositorio.unifesp.br/handle/11600/37648

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