Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups

Oligofructose supplementation during pregnancy and lactation impairs offspring development and alters the intestinal properties of 21-d-old pups

Author Mennitti, Lais Vales Autor UNIFESP Google Scholar
Oyama, Lila Missae Autor UNIFESP Google Scholar
Oliveira, Juliana Lopez de Autor UNIFESP Google Scholar
Hachul, Ana Claudia Losinskas Autor UNIFESP Google Scholar
Santamarina, Aline Boveto Autor UNIFESP Google Scholar
Santana, Aline Alves de Autor UNIFESP Google Scholar
Okuda, Marcos Hiromu Autor UNIFESP Google Scholar
Ribeiro, Eliane Beraldi Autor UNIFESP Google Scholar
Nascimento, Claudia Maria da Penha Oller do Autor UNIFESP Google Scholar
Pisani, Luciana Pellegrini Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Background: Previously, we showed that the intake of trans fatty acids during pregnancy and lactation triggers a pro-inflammatory status in the offspring. On the other hand, prebiotics can alter the intestinal environment, reducing serum lipopolysaccharides (LPS) concentrations. This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring.Methods: On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF). Diets were maintained during pregnancy and lactation. At birth, 7th, 14th and 21th, pups were weighed and length was measured. Serum concentrations of LPS and free fatty acids (FFA) were performed by specific kits. Bacterial DNA present in faeces was determined by real-time PCR. Data were expressed as mean +/- standard error of the mean and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures. p < 0.05 was considered significant.Results: We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp. on faeces of the CF group in relation to C group.Conclusion: in conclusion, dam's diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.
Keywords Hydrogenated vegetable fat
Oligofructose
Corporal composition
Lipopolysaccharides
Lactobacillus spp
Pregnancy
Lactation
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2011/19426-2
Date 2014-02-05
Published in Lipids in Health and Disease. London: Biomed Central Ltd, v. 13, 10 p., 2014.
ISSN 1476-511X (Sherpa/Romeo, impact factor)
Publisher Biomed Central Ltd
Extent 10
Origin http://dx.doi.org/10.1186/1476-511X-13-26
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000333647200002
URI http://repositorio.unifesp.br/handle/11600/37428

Show full item record




File

Name: WOS000333647200002.pdf
Size: 669.3Kb
Format: PDF
Description:
Open file

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account