Genotoxicity of Endosseous Implants Using Two Cellular Lineages in Vitro

Genotoxicity of Endosseous Implants Using Two Cellular Lineages in Vitro

Author Matsumoto, Mariza Akemi Google Scholar
Nary Filho, Hugo Google Scholar
Ferrari, Raquel Google Scholar
Fernandes, Kristianne Google Scholar
Renno, Ana Claudia Muniz Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Institution Univ Sagrado Coracao
Universidade de São Paulo (USP)
Univ Nove de Julho
Universidade Federal de São Paulo (UNIFESP)
Abstract The genotoxic potential of corrosion eluates obtained from a single dental implant using murine fibroblasts or osteoblasts cells in vitro by the single-cell gel (comet) assay was examined. A single commercially available dental implant (Biotechnology) was eluted in a solution consisting of equal amounts of acetic acid and sodium chloride (0.1 M) for 1, 3, 7, 14, and 21 days. Murine fibroblast or osteoblast cultures were then exposed to all corrosion eluates obtained from endosseous dental implants for 30 minutes at 37 degrees C. the results suggest that none of the eluates produced genotoxic changes in murine fibroblasts regardless of the length of exposure to the eluate. Similarly, no genotoxicity was found in osteoblasts. the results suggest that the dental implant eluates tested in this study did not induce genetic damage as depicted by the single-cell gel (comet) assay. Because DNA damage is an important event during oncogenesis, this study represents a relevant contribution to estimate the real risks to the cellular system induced by the corrosion products of a dental implant.
Keywords Endosseous implant
Genotoxicity
Mouse fibroblast cells
Osteoblasts
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2014-02-01
Published in Journal of Oral Implantology. Lawrence: Allen Press Inc, v. 40, n. 1, p. 25-30, 2014.
ISSN 0160-6972 (Sherpa/Romeo, impact factor)
Publisher Allen Press Inc
Extent 25-30
Origin https://dx.doi.org/10.1563/AAID-JOI-D-10-00112
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000333657100006
URI https://repositorio.unifesp.br/handle/11600/37419

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