Differential role of the estrogen receptors ESR1 and ESR2 on the regulation of proteins involved with proliferation and differentiation of Sertoli cells from 15-day-old rats

Differential role of the estrogen receptors ESR1 and ESR2 on the regulation of proteins involved with proliferation and differentiation of Sertoli cells from 15-day-old rats

Author Lucas, Thais F. G. Autor UNIFESP Google Scholar
Lazari, Maria Fatima M. Autor UNIFESP Google Scholar
Porto, Catarina S. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The aim of the present study was to investigate the role of each estrogen receptors on the regulation of proteins involved with proliferation and differentiation of Sertoli cells from 15-day-old rats. Activation of ESR1 by 17 beta-estradiol (E2) and ESR1-selective agonist PPT increased CCND1 expression, and this effect was dependent on NF-kB activation. E2 and the ESR2-selective agonist DPN, but not PPT, increased, in a PI3K and CREB-dependent manner, the expression of CDKN1B and the transcription factors GATA-1 and DMRT1. Analyzing the expression of ESR1 and ESR2 in different stages of development of Sefton cells, we observed that the ESR1/ESR2 ratio decreased with age, and this ratio seems to be important to determine the end of cell proliferation and the start of cell differentiation. in Sertoli cells from 15-day-old rats, the ESR1/ESR2 ratio favors the effect of ESR1 and the activation of this receptor increased [Methyl-31-I]thymidine incorporation. We propose that in Sertoli cells from 15-day-old rats E2 modulates Sertoli cell proliferation through ESR1/NF-kappa B-mediated increase of CCND1, and cell cycle exit and differentiation through ESR2/CREB-mediated increase of CDKN1B, GATA-1 and DMRT1. the present study reinforces the important role of estrogen for normal testis development. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
Keywords Estrogen receptors
Sertoli cells
CCND1
CDKN1B
GATA-1
DMRT1
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2010/52306-8
Date 2014-01-25
Published in Molecular and Cellular Endocrinology. Clare: Elsevier B.V., v. 382, n. 1, p. 84-96, 2014.
ISSN 0303-7207 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 84-96
Origin http://dx.doi.org/10.1016/j.mce.2013.09.015
Access rights Closed access
Type Article
Web of Science ID WOS:000330421600010
URI http://repositorio.unifesp.br/handle/11600/37328

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