A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential

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dc.contributor.author Brito, Adriana S.
dc.contributor.author Cavalcante, Romulo S.
dc.contributor.author Palhares, Lais C. G. F.
dc.contributor.author Hughes, Ashley J.
dc.contributor.author Andrade, Giulianna P. V.
dc.contributor.author Yates, Edwin Alexander [UNIFESP]
dc.contributor.author Nader, Helena Bonciani [UNIFESP]
dc.contributor.author Lima, Marcelo Andrade de [UNIFESP]
dc.contributor.author Chavante, Suely F.
dc.date.accessioned 2016-01-24T14:35:08Z
dc.date.available 2016-01-24T14:35:08Z
dc.date.issued 2014-01-02
dc.identifier http://dx.doi.org/10.1016/j.carbpol.2013.08.063
dc.identifier.citation Carbohydrate Polymers. Oxford: Elsevier B.V., v. 99, p. 372-378, 2014.
dc.identifier.issn 0144-8617
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/37305
dc.description.abstract The structural characterization and the anticoagulant potential of a novel heparin/heparan sulfate-like compound from the heads of Litopenaeus vannamei shrimp are described. While it is distinct from either heparin or heparan sulfate, enzymatic depolymerization and nuclear magnetic resonance spectroscopy analyses revealed that this molecule does share some structural features with heparin, such as the high degree of N- and 6-0-sulfation and minor N-acetylation, and with heparan sulfate, in the glucuronic acid content. Its ability to stabilize human antithrombin explains its significant anticoagulant activity in aPTT and Factor-Xa inhibition assays. Interestingly, in contrast to mammalian heparin, the shrimp compound displayed negligible hemorrhagic effect. Together, these findings have particular interest since they reveal a novel molecule with significant anti-Xa activity coupled with low bleeding effects which make the shrimp heparin/HS-like compound a potential alternative for mammalian heparin. (C) 2013 Elsevier B.V. All rights reserved. en
dc.description.sponsorship PPG.BIOQ (Programa de Pos-graduagao em Bioquimica/UFRN)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Lais
dc.format.extent 372-378
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Carbohydrate Polymers
dc.rights Acesso aberto
dc.subject Heparin en
dc.subject Heparan sulfate en
dc.subject Hemorrhagic activity en
dc.subject Antithrombin en
dc.subject Anticoagulant en
dc.subject Thermostabilization en
dc.title A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Univ Fed Rio Grande do Norte
dc.contributor.institution Univ Liverpool
dc.contributor.institution Diamond Light Source Ltd
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Fed Rio Grande do Norte, Dept Bioquim, BR-59072970 Natal, RN, Brazil
dc.description.affiliation Univ Fed Rio Grande do Norte, Fac Ciencias Saude Trairi, Santa Cruz, RN, Brazil
dc.description.affiliation Univ Liverpool, Dept Struct & Chem Biol, Liverpool L69 7ZB, Merseyside, England
dc.description.affiliation Diamond Light Source Ltd, Didcot OX11 0DE, Oxon, England
dc.description.affiliation Universidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.identifier.file WOS000329256600048.pdf
dc.identifier.doi 10.1016/j.carbpol.2013.08.063
dc.description.source Web of Science
dc.identifier.wos WOS:000329256600048


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