A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential

A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential

Author Brito, Adriana S. Google Scholar
Cavalcante, Romulo S. Google Scholar
Palhares, Lais C. G. F. Google Scholar
Hughes, Ashley J. Google Scholar
Andrade, Giulianna P. V. Google Scholar
Yates, Edwin Alexander Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Lima, Marcelo Andrade de Autor UNIFESP Google Scholar
Chavante, Suely F. Google Scholar
Institution Univ Fed Rio Grande do Norte
Univ Liverpool
Diamond Light Source Ltd
Universidade Federal de São Paulo (UNIFESP)
Abstract The structural characterization and the anticoagulant potential of a novel heparin/heparan sulfate-like compound from the heads of Litopenaeus vannamei shrimp are described. While it is distinct from either heparin or heparan sulfate, enzymatic depolymerization and nuclear magnetic resonance spectroscopy analyses revealed that this molecule does share some structural features with heparin, such as the high degree of N- and 6-0-sulfation and minor N-acetylation, and with heparan sulfate, in the glucuronic acid content. Its ability to stabilize human antithrombin explains its significant anticoagulant activity in aPTT and Factor-Xa inhibition assays. Interestingly, in contrast to mammalian heparin, the shrimp compound displayed negligible hemorrhagic effect. Together, these findings have particular interest since they reveal a novel molecule with significant anti-Xa activity coupled with low bleeding effects which make the shrimp heparin/HS-like compound a potential alternative for mammalian heparin. (C) 2013 Elsevier B.V. All rights reserved.
Keywords Heparin
Heparan sulfate
Hemorrhagic activity
Antithrombin
Anticoagulant
Thermostabilization
Language English
Sponsor PPG.BIOQ (Programa de Pos-graduagao em Bioquimica/UFRN)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Lais
Date 2014-01-02
Published in Carbohydrate Polymers. Oxford: Elsevier B.V., v. 99, p. 372-378, 2014.
ISSN 0144-8617 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 372-378
Origin http://dx.doi.org/10.1016/j.carbpol.2013.08.063
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000329256600048
URI http://repositorio.unifesp.br/handle/11600/37305

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