Organization and Behavior of the Synaptonemal Complex during Achiasmatic Meiosis of Four Buthid Scorpions

Organization and Behavior of the Synaptonemal Complex during Achiasmatic Meiosis of Four Buthid Scorpions

Author Schneider, Marielle C. Autor UNIFESP Google Scholar
Mattos, Viviane F. Google Scholar
Carvalho, Leonardo S. Google Scholar
Cella, Doralice M. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Estadual Paulista UNESP
Univ Fed Piaui UFPI
Abstract Testicular cells of 4 buthid scorpions, Rhopalurus agamemnon (2n = 28), R. rochai (2n = 28), Tityus bahiensis (2n = 6), and T. fasciolatus (2n = 14), which show different types of chromosomal configurations in meiosis I, were subjected to cellular microspreading in order to (1) obtain knowledge about the organization and behavior of the synaptonemal complex (SC), and (2) acquire data about the mechanisms responsible for inter-and intraindividual chromosomal variation within Buthidae. Ultrastructural analysis of microspread nuclei revealed SCs with a well-preserved structure until late substages of prophase I, but did not detect kinetochore plates and recombination nodules. Pachytene cells of R. agamemnon, R. rochai and T. bahiensis exhibited single and unsynapsed axes continuous with totally synapsed SCs, indicating the occurrence of heterozygous chromosomal rearrangements. Although chromosome chains were not observed in T. fasciolatus, the presence of gaps and interlocks points out that this species also carries heterozygous rearrangements, involving a small chromosome segment. Especially in R. rochai, the cellular microspreading analysis was useful to clarify the origin of inter-and intraindividual variation in the number of bivalent-like elements and in the number of chromosomes involved in multivalent associations. It was found that more chromosomes were involved in rearrangements than previously established through investigations using light microscopy alone. (C) 2015 S. Karger AG, Basel
Keywords Chromosomal rearrangements
Holocentric chromosomes
Multivalent associations
Rhopalurus
Tityus
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Instituto Brasileiro de Meio Ambiente e dos Recursos Naturais Renovaveis (IBAMA)
Instituto Chico Mendes de Conservacao e Biodiversidade (ICMBio)
Grant number FAPESP: 2010/142262
FAPESP: 2011/21643-1
CNPq: 478630/2010-7
Instituto Chico Mendes de Conservacao e Biodiversidade (ICMBio): 25471-1
Instituto Chico Mendes de Conservacao e Biodiversidade (ICMBio): 25472-1
Instituto Chico Mendes de Conservacao e Biodiversidade (ICMBio): 15157-1
Date 2014-01-01
Published in Cytogenetic and Genome Research. Basel: Karger, v. 144, n. 4, p. 341-347, 2014.
ISSN 1424-8581 (Sherpa/Romeo, impact factor)
Publisher Karger
Extent 341-347
Origin http://dx.doi.org/10.1159/000375388
Access rights Closed access
Type Article
Web of Science ID WOS:000353302800013
URI http://repositorio.unifesp.br/handle/11600/37293

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