Liquid biopsy of atherosclerosis using protoporphyrin IX as a biomarker

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dc.contributor.author Silva, Monica Nascimento da [UNIFESP]
dc.contributor.author Sicchieri, Leticia Bonfante
dc.contributor.author Silva, Flávia Rodrigues de Oliveira
dc.contributor.author Andrade, Maira Franco
dc.contributor.author Courrol, Lilia Coronato [UNIFESP]
dc.date.accessioned 2016-01-24T14:35:04Z
dc.date.available 2016-01-24T14:35:04Z
dc.date.issued 2014-01-01
dc.identifier http://dx.doi.org/10.1039/c3an01945d
dc.identifier.citation Analyst. Cambridge: Royal Soc Chemistry, v. 139, n. 6, p. 1383-1388, 2014.
dc.identifier.issn 0003-2654
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/37254
dc.description.abstract Protoporphyrin IX (PPIX), a derivative of hematoporphyrin, can accumulate in rapidly growing tissues, including tumors and atherosclerotic plaques. the objective of this study is to employ PPIX fluorescence to detect the changes in blood caused by the formation of atheromatous plaques in arteries; this measurement can function as a liquid biopsy. for this purpose twenty four rabbits were randomly divided into groups: control group (CG) - fed with a normal diet, and an experimental group (EG) - fed with a hypercholesterolemic diet (1% cholesterol). Blood samples were collected before (0 time) and after 22, 43, 64 days to measure biochemical factors. the aortas were removed after 22, 43 and 64 days to assess the atherosclerotic plaques. PPIX was extracted from the blood and fluorescence was measured in the 550-750 nm range from samples that were excited at 405 nm. Aminolevulinic acid (ALA) was administered intravenously to increase the PPIX fluorescence intensity in the arteries and consequently in the liquid biopsy of the atherosclerotic plaques. the results have shown that the PPIX fluorescence increased as the atheromatous plaques grew. the aorta fluorescence and the PPIX fluorescence increased in the animals in the experimental group that received ALA. PPIX that accumulates in atheromatous plaques transfers to the blood and can be analyzed by extracting porphyrin from total blood. Therefore, this method can aid in the early diagnosis of atherosclerosis with high sensitivity. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent 1383-1388
dc.language.iso eng
dc.publisher Royal Soc Chemistry
dc.relation.ispartof Analyst
dc.rights Acesso restrito
dc.title Liquid biopsy of atherosclerosis using protoporphyrin IX as a biomarker en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Ctr Lasers & Aplicacoes
dc.contributor.institution Ctr Ciencia & Tecnol Mat
dc.description.affiliation Universidade Federal de São Paulo, Lab Lasers & Opt Biomed Aplicada LOBA, Inst Ciencias Ambientais Quim & Farmaceut ICAQF, Dept Ciencias Exatas & Terra DCET, BR-09972270 Diadema, SP, Brazil
dc.description.affiliation Ctr Lasers & Aplicacoes, Inst Pesquisas Energet & Nucl, São Paulo, Brazil
dc.description.affiliation Ctr Ciencia & Tecnol Mat, Inst Pesquisas Energet & Nucl, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Lab Lasers & Opt Biomed Aplicada LOBA, Inst Ciencias Ambientais Quim & Farmaceut ICAQF, Dept Ciencias Exatas & Terra DCET, BR-09972270 Diadema, SP, Brazil
dc.description.sponsorshipID FAPESP: 2010/016544-1
dc.identifier.doi 10.1039/c3an01945d
dc.description.source Web of Science
dc.identifier.wos WOS:000331867500015



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